microRNA let-7i-5p mediates the relationship between muscle fat infiltration and neck pain disability following motor vehicle collision: a preliminary study

James M. Elliott; Cathleen A. Rueckeis; Yue Pan; Todd B. Parrish; David M. Walton; Sarah D. Linnstaedt

doi:10.1038/s41598-021-82734-y

microRNA let-7i-5p mediates the relationship between muscle fat infiltration and neck pain disability following motor vehicle collision: a preliminary study

James M. Elliott, Cathleen A. Rueckeis, Yue Pan, Todd B. Parrish, David M. Walton, Sarah D. Linnstaedt^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Persistent neck-pain disability (PNPD) is common following traumatic stress exposures such as motor vehicle collision (MVC). Substantial literature indicates that fat infiltration into neck muscle (MFI) is associated with post-MVC PNPD. However, little is known about the molecular mediators underlying this association. In the current study, we assessed whether microRNA expression signatures predict PNPD and whether microRNA mediate the relationship between neck MFI and PNPD. A nested cohort of 43 individuals from a longitudinal study of MVC survivors, who provided blood (PAXgene RNA) and underwent magnetic resonance imaging (MRI), were included in the current study. Peritraumatic microRNA expression levels were quantified via small RNA sequencing, neck MFI via MRI, and PNPD via the Neck Disability Index two-weeks, three-months, and twelve-months following MVC. Repeated measures regression models were used to assess the relationship between microRNA and PNPD and to perform mediation analyses. Seventeen microRNA predicted PNPD following MVC. One microRNA, let-7i-5p, mediated the relationship between neck MFI and PNPD. Peritraumatic blood-based microRNA expression levels predict PNPD following MVC and let-7i-5p might contribute to the underlying effects of neck MFI on persistent disability. In conclusion, additional studies are needed to validate this finding.

Original language	English (US)
Article number	3140
Journal	Scientific reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-82734-y
State	Published - Dec 2021

Funding

The authors would like to acknowledge the study participants for their time and interest in participating in this study, Marie Wasielewski for her incredible leadership in participant recruitment and data collection, and Samuel McLean for his advice and collaborative brainstorming in study design stage of this project. JME and TBP are supported by NIH R01HD079076. SDL is supported by NIH K01AR071504. This work originated at Northwestern University, Feinberg School of Medicine, Chicago, IL USA and was continued at University of North Carolina at Chapel Hill, Chapel Hill, NC USA. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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title = "microRNA let-7i-5p mediates the relationship between muscle fat infiltration and neck pain disability following motor vehicle collision: a preliminary study",

abstract = "Persistent neck-pain disability (PNPD) is common following traumatic stress exposures such as motor vehicle collision (MVC). Substantial literature indicates that fat infiltration into neck muscle (MFI) is associated with post-MVC PNPD. However, little is known about the molecular mediators underlying this association. In the current study, we assessed whether microRNA expression signatures predict PNPD and whether microRNA mediate the relationship between neck MFI and PNPD. A nested cohort of 43 individuals from a longitudinal study of MVC survivors, who provided blood (PAXgene RNA) and underwent magnetic resonance imaging (MRI), were included in the current study. Peritraumatic microRNA expression levels were quantified via small RNA sequencing, neck MFI via MRI, and PNPD via the Neck Disability Index two-weeks, three-months, and twelve-months following MVC. Repeated measures regression models were used to assess the relationship between microRNA and PNPD and to perform mediation analyses. Seventeen microRNA predicted PNPD following MVC. One microRNA, let-7i-5p, mediated the relationship between neck MFI and PNPD. Peritraumatic blood-based microRNA expression levels predict PNPD following MVC and let-7i-5p might contribute to the underlying effects of neck MFI on persistent disability. In conclusion, additional studies are needed to validate this finding.",

author = "Elliott, {James M.} and Rueckeis, {Cathleen A.} and Yue Pan and Parrish, {Todd B.} and Walton, {David M.} and Linnstaedt, {Sarah D.}",

note = "Funding Information: The authors would like to acknowledge the study participants for their time and interest in participating in this study, Marie Wasielewski for her incredible leadership in participant recruitment and data collection, and Samuel McLean for his advice and collaborative brainstorming in study design stage of this project. JME and TBP are supported by NIH R01HD079076. SDL is supported by NIH K01AR071504. This work originated at Northwestern University, Feinberg School of Medicine, Chicago, IL USA and was continued at University of North Carolina at Chapel Hill, Chapel Hill, NC USA. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1038/s41598-021-82734-y",

language = "English (US)",

volume = "11",

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T1 - microRNA let-7i-5p mediates the relationship between muscle fat infiltration and neck pain disability following motor vehicle collision

T2 - a preliminary study

AU - Elliott, James M.

AU - Rueckeis, Cathleen A.

AU - Pan, Yue

AU - Parrish, Todd B.

AU - Walton, David M.

AU - Linnstaedt, Sarah D.

N1 - Funding Information: The authors would like to acknowledge the study participants for their time and interest in participating in this study, Marie Wasielewski for her incredible leadership in participant recruitment and data collection, and Samuel McLean for his advice and collaborative brainstorming in study design stage of this project. JME and TBP are supported by NIH R01HD079076. SDL is supported by NIH K01AR071504. This work originated at Northwestern University, Feinberg School of Medicine, Chicago, IL USA and was continued at University of North Carolina at Chapel Hill, Chapel Hill, NC USA. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: © 2021, The Author(s).

PY - 2021/12

Y1 - 2021/12

N2 - Persistent neck-pain disability (PNPD) is common following traumatic stress exposures such as motor vehicle collision (MVC). Substantial literature indicates that fat infiltration into neck muscle (MFI) is associated with post-MVC PNPD. However, little is known about the molecular mediators underlying this association. In the current study, we assessed whether microRNA expression signatures predict PNPD and whether microRNA mediate the relationship between neck MFI and PNPD. A nested cohort of 43 individuals from a longitudinal study of MVC survivors, who provided blood (PAXgene RNA) and underwent magnetic resonance imaging (MRI), were included in the current study. Peritraumatic microRNA expression levels were quantified via small RNA sequencing, neck MFI via MRI, and PNPD via the Neck Disability Index two-weeks, three-months, and twelve-months following MVC. Repeated measures regression models were used to assess the relationship between microRNA and PNPD and to perform mediation analyses. Seventeen microRNA predicted PNPD following MVC. One microRNA, let-7i-5p, mediated the relationship between neck MFI and PNPD. Peritraumatic blood-based microRNA expression levels predict PNPD following MVC and let-7i-5p might contribute to the underlying effects of neck MFI on persistent disability. In conclusion, additional studies are needed to validate this finding.

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microRNA let-7i-5p mediates the relationship between muscle fat infiltration and neck pain disability following motor vehicle collision: a preliminary study

Abstract

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