Abstract
Tomography using diffracted x-rays produces reconstructions mapping quantities including crystal lattice parameter(s), crystallite size and crystallographic texture; this information is quite different from that obtained with absorption contrast or phase contrast. Diffraction tomography can be performed using energy dispersive diffraction (EDD) and polychromatic synchrotron x-radiation. In EDD, different, properly-oriented Bragg planes diffract different x-ray energies; these intensities are measured by an energy sensitive detector. A pencil beam defines the irradiated volume, and a collimator before the energy sensitive detector selects which portion of the irradiated column is sampled at any one time. A 3D map is assembled by translating the specimen along X, Y and Z axes. This paper reports results of 3D mapping of the integrated intensity of several reflections from the bioapatite in the mineralized cartilage centrum of a blue shark. The multiple detector EDD system at 6-BM-B, the Advanced Photon Source was used to map an entire blue shark centrum. The shark centrum consists of a double cone structure (corpora calcerea) supported by the intermedialia consisting of four wedges. The integrated intensities of the c-axis reflection and of a reflection with no c-axis component reveals the bioapatite within the cone wall is oriented with its c-axes lateral, i.e., perpendicular to the axis of the backbone, whereas the bioapatite within the wedges is oriented with its c-axes axial. Results of absorption microCT (laboratory and synchrotron) and x-ray excited x-ray fluorescence mapping are included to provide higher resolution data of the structures underlying the EDD maps. Application of EDD tomography to 3D mapping of large specimens promises to add to the understanding of other mineralized tissue samples which cannot be sectioned.
Original language | English (US) |
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Title of host publication | Developments in X-Ray Tomography XIII |
Editors | Bert Muller, Ge Wang |
Publisher | SPIE |
ISBN (Electronic) | 9781510645189 |
DOIs | |
State | Published - 2021 |
Event | Developments in X-Ray Tomography XIII 2021 - San Diego, United States Duration: Aug 1 2021 → Aug 5 2021 |
Publication series
Name | Proceedings of SPIE - The International Society for Optical Engineering |
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Volume | 11840 |
ISSN (Print) | 0277-786X |
ISSN (Electronic) | 1996-756X |
Conference
Conference | Developments in X-Ray Tomography XIII 2021 |
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Country/Territory | United States |
City | San Diego |
Period | 8/1/21 → 8/5/21 |
Funding
The lab microCT data were collected at the Duke University Shared Materials Instrumentation Facility (SMIF), a member of the North Carolina Research Triangle Nanotechnology Network (RTNN), which is supported by the National Science Foundation (Grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI); we thank Justin Gladman for his support of this imaging. Beamline 6BM-B, APS, is supported by COMPRES, the Consortium for Materials Properties Research in Earth Sciences under NSF Cooperative Agreement EAR-1661511. This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility, operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357.
Keywords
- Bioapatite
- Energy dispersive diffraction
- MicroCT
- Mineralized cartilage
- Shark
- Vertebra
- X-ray fluorescence
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering