Microwave decondensation and codenaturation: A new methodology to maximize FISH data from donors with very low concentrations of sperm

E. Ko, A. Rademaker, R. Martin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

To increase analyzable sperm numbers when dealing with semen samples from donors with extremely low quantities of sperm, a microwave decondensation/codenaturation technique for fluorescence in situ hybridization (FISH) was developed and results were compared to those obtained from conventional FISH techniques. There was no significant difference between the frequencies of disomy and diploidy obtained using the two techniques.

Original languageEnglish (US)
Pages (from-to)143-145
Number of pages3
JournalCytogenetics and Cell Genetics
Volume95
Issue number3-4
StatePublished - Jun 26 2002

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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