Microwave decondensation and codenaturation: A new methodology to maximize FISH data from donors with very low concentrations of sperm

E. Ko; A. Rademaker; R. Martin

Microwave decondensation and codenaturation: A new methodology to maximize FISH data from donors with very low concentrations of sperm

E. Ko, A. Rademaker, R. Martin^*

^*Corresponding author for this work

Preventive Medicine

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

To increase analyzable sperm numbers when dealing with semen samples from donors with extremely low quantities of sperm, a microwave decondensation/codenaturation technique for fluorescence in situ hybridization (FISH) was developed and results were compared to those obtained from conventional FISH techniques. There was no significant difference between the frequencies of disomy and diploidy obtained using the two techniques.

Original language	English (US)
Pages (from-to)	143-145
Number of pages	3
Journal	Cytogenetics and Cell Genetics
Volume	95
Issue number	3-4
State	Published - Jun 26 2002

ASJC Scopus subject areas

Genetics
Cell Biology

Cite this

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title = "Microwave decondensation and codenaturation: A new methodology to maximize FISH data from donors with very low concentrations of sperm",

abstract = "To increase analyzable sperm numbers when dealing with semen samples from donors with extremely low quantities of sperm, a microwave decondensation/codenaturation technique for fluorescence in situ hybridization (FISH) was developed and results were compared to those obtained from conventional FISH techniques. There was no significant difference between the frequencies of disomy and diploidy obtained using the two techniques.",

author = "E. Ko and A. Rademaker and R. Martin",

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T2 - A new methodology to maximize FISH data from donors with very low concentrations of sperm

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AU - Rademaker, A.

AU - Martin, R.

PY - 2002/6/26

Y1 - 2002/6/26

N2 - To increase analyzable sperm numbers when dealing with semen samples from donors with extremely low quantities of sperm, a microwave decondensation/codenaturation technique for fluorescence in situ hybridization (FISH) was developed and results were compared to those obtained from conventional FISH techniques. There was no significant difference between the frequencies of disomy and diploidy obtained using the two techniques.

AB - To increase analyzable sperm numbers when dealing with semen samples from donors with extremely low quantities of sperm, a microwave decondensation/codenaturation technique for fluorescence in situ hybridization (FISH) was developed and results were compared to those obtained from conventional FISH techniques. There was no significant difference between the frequencies of disomy and diploidy obtained using the two techniques.

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IS - 3-4

ER -

Microwave decondensation and codenaturation: A new methodology to maximize FISH data from donors with very low concentrations of sperm

Abstract

ASJC Scopus subject areas

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