Migration and differentiation of human bone marrow mesenchymal stem cells in the rat brain

Ling Ling Hou, Min Zheng, Dong Mei Wang, Hong Feng Yuan, Hai Min Li, Lin Chen, Ci Xian Bai, Yong Zhang, Xue Tao Pei*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Bone marrow mesenchymal stem cells (MSCs) are multipotent tissue stem cells that can be induced in vitro to differentiate into a variety of cells such as osteoblasts, chondrocytes and adipocytes. MSCs are useful vehicles for both cell and gene therapy for a variety of diseases. Here, we injected human MSCs with enhanced green fluorescent protein (EGFP) into the striatum of Parkinson disease (PD) rat and examined their survival, migration, differentiation, and the behavior changes in PD rats, which will provide a theoretical foundation and technical method for clinic PD therapy by stem cells. The results showed that human bone marrow MSCs can survive in rat brain for a long time (exceeding 70 d). MSCs were found in multiple areas of the rat brain including the striatum, the corpus callosum, contralateral cortex and even the brain vascular wall. Immunocytochemical staining suggested that implanted cells expressed human neurofilament (NF), neuron-specific enolase (NSE) and glial fibrillary acid protein (GFAP). At the same time, remission in abnormal behavior of the PD rats appeared. Rotation scores decreased gradually from 8. 86 ± 2. 09 r/min pre-transplantation to 4. 87 ± 2. 06 r/min 90 d post-transplantation (statistic result showed P < 0. 05).

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalActa Physiologica Sinica
Volume55
Issue number2
StatePublished - Apr 25 2003
Externally publishedYes

Keywords

  • Brain
  • Differentiation
  • Mesenchymal stem cells
  • Migration
  • Rat

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint

Dive into the research topics of 'Migration and differentiation of human bone marrow mesenchymal stem cells in the rat brain'. Together they form a unique fingerprint.

Cite this