Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM-1

Fang Liao, Hanh K. Huynh, Ana Eiroa, Tricia Greene, Elizabeth Polizzi, William A. Muller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

211 Scopus citations

Abstract

During the inflammatory response, the adhesion molecule PECAM plays a crucial role in transendothehal migration, the passage of leukocytes across endothelium. We report here an additional role for PECAM in the subsequent migration of monocytes through the subendothelial extraceHular matrix. PECAM has six immunoglobulin (Ig) superfamily domains. Monoclonal antibodies whose epitopes map to domains 1 and/or 2 selectively block monocyte migration through the endothelial junction, whereas those that map to domain 6 block only the migration through the extracellular matrix, trapping the monocyte between the endothelium and its basal lamina. Therefore, transendothelial migration (diapedesis) and passage through extracellnlar matrix (interstitial migration) are distinct and separable phases of monocyte emigration. Furthermore, distinct and separate Ig domains of PECAM are involved in mediating these two steps.

Original languageEnglish (US)
Pages (from-to)1337-1343
Number of pages7
JournalJournal of Experimental Medicine
Volume182
Issue number5
DOIs
StatePublished - Nov 1 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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