Mimicking stem cell niches to increase stem cell expansion

Shara M. Dellatore*, A. Sofia Garcia, William M Miller

*Corresponding author for this work

Research output: Contribution to journalReview article

168 Scopus citations

Abstract

Niches regulate lineage-specific stem cell self-renewal versus differentiation in vivo and are composed of supportive cells and extracellular matrix components arranged in a three-dimensional topography of controlled stiffness in the presence of oxygen and growth factor gradients. Mimicking stem cell niches in a defined manner will facilitate production of the large numbers of stem cells needed to realize the promise of regenerative medicine and gene therapy. Progress has been made in mimicking components of the niche. Immobilizing cell-associated Notch ligands increased the self-renewal of hematopoietic (blood) stem cells. Culture on a fibrous scaffold that mimics basement membrane texture increased the expansion of hematopoietic and embryonic stem cells. Finally, researchers have created intricate patterns of cell-binding domains and complex oxygen gradients.

Original languageEnglish (US)
Pages (from-to)534-540
Number of pages7
JournalCurrent Opinion in Biotechnology
Volume19
Issue number5
DOIs
StatePublished - Oct 1 2008

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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