Abstract
Off-line miniaturized nanospray formats for electrospray ionization mass spectrometry enable the routine identification of femtomole quantities of protein or peptide. Even greater strides have been achieved using on-line miniaturized nanobore LC-MS/MS. In either off- or on-line methods for protein analysis, samples are typically isolated and digested enzymatically, with MS/MS analysis of the peptide fragments in a bottom-up approach. Biologically relevant (structure/function) information often demands 100% sequence coverage and this may be obtained by the analysis of intact proteins by MS with a top-down methodology. Proteome wide success with LC-MS based methods will require the development of novel miniaturized approaches for sample preparation along with new tools for bioinformatics. As these miniaturized formats continue to power proteomics analysis, cross-over applications in LC-MS will undoubtedly result that range from drug discovery to development.
Original language | English (US) |
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Title of host publication | Integrated Strategies for Drug Discovery Using Mass Spectrometry |
Publisher | John Wiley & Sons, Inc. |
Pages | 1-26 |
Number of pages | 26 |
ISBN (Print) | 047146127X, 9780471461272 |
DOIs | |
State | Published - Jan 27 2005 |
Keywords
- Bottom-up proteomics
- Capillary chromatography
- Electrospray ionization
- Mass spectrometry
- Microscale separation
- Multidimensional separation
- Nanobore chromatography
- Nanoelectrospray
- Nanospray
- Peptide identification
- Proteomics
- Sample trapping
- Top-down proteomics
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)
- General Biochemistry, Genetics and Molecular Biology