Miniaturized Formats for Efficient Liquid Chromatography Mass Spectrometry-Based Proteomics and Therapeutic Development

Gary A. Valaskovic*, Neil L. Kelleher

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Off-line miniaturized nanospray formats for electrospray ionization mass spectrometry enable the routine identification of femtomole quantities of protein or peptide. Even greater strides have been achieved using on-line miniaturized nanobore LC-MS/MS. In either off- or on-line methods for protein analysis, samples are typically isolated and digested enzymatically, with MS/MS analysis of the peptide fragments in a bottom-up approach. Biologically relevant (structure/function) information often demands 100% sequence coverage and this may be obtained by the analysis of intact proteins by MS with a top-down methodology. Proteome wide success with LC-MS based methods will require the development of novel miniaturized approaches for sample preparation along with new tools for bioinformatics. As these miniaturized formats continue to power proteomics analysis, cross-over applications in LC-MS will undoubtedly result that range from drug discovery to development.

Original languageEnglish (US)
Title of host publicationIntegrated Strategies for Drug Discovery Using Mass Spectrometry
PublisherJohn Wiley & Sons, Inc.
Pages1-26
Number of pages26
ISBN (Print)047146127X, 9780471461272
DOIs
StatePublished - Jan 27 2005

Keywords

  • Bottom-up proteomics
  • Capillary chromatography
  • Electrospray ionization
  • Mass spectrometry
  • Microscale separation
  • Multidimensional separation
  • Nanobore chromatography
  • Nanoelectrospray
  • Nanospray
  • Peptide identification
  • Proteomics
  • Sample trapping
  • Top-down proteomics

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Valaskovic, G. A., & Kelleher, N. L. (2005). Miniaturized Formats for Efficient Liquid Chromatography Mass Spectrometry-Based Proteomics and Therapeutic Development. In Integrated Strategies for Drug Discovery Using Mass Spectrometry (pp. 1-26). John Wiley & Sons, Inc.. https://doi.org/10.1002/0471721034.ch1