MIR-1343 attenuates pathways of fibrosis by targeting the TGF-ß receptors

Lindsay R. Stolzenburg, Sarah Wachtel, Hong Dang, Ann Harris*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Irreversible respiratory obstruction resulting from progressive airway damage, inflammation and fibrosis is a feature of several chronic respiratory diseases, including cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD). The cytokine transforming growth factor ß (TGF-ß) has a pivotal role in promoting lung fibrosis and is implicated in respiratory disease severity. In the present study, we show that a previously uncharacterized miRNA, miR- 1343, reduces the expression of both TGF-ß receptor 1 and 2 by directly targeting their 3'-UTRs. After TGF-ß exposure, elevated intracellular miR-1343 significantly decreases levels of activated TGF-ß effector molecules, pSMAD2 (phosphorylated SMAD2) and pSMAD3 (phosphorylated SMAD3), when compared with a non-targeting control miRNA. As a result, the abundance of fibrotic markers is reduced, cell migration into a scratch wound impaired and epithelial-to-mesenchymal transition (EMT) repressed. Mature miR-1343 is readily detected in human neutrophils andHL-60 cells and is activated in response to stress in A549 lung epithelial cells. miR-1343 may have direct therapeutic applications in fibrotic lung disease.

Original languageEnglish (US)
Pages (from-to)245-256
Number of pages12
JournalBiochemical Journal
Volume473
Issue number3
DOIs
StatePublished - Feb 1 2016

Keywords

  • Fibrosis
  • Lung
  • MicroRNA
  • Transforming growth factor ß

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'MIR-1343 attenuates pathways of fibrosis by targeting the TGF-ß receptors'. Together they form a unique fingerprint.

Cite this