Misexpression of miR-196a induces eye anomaly in Xenopus laevis

Rong Qiu, Ying Liu*, Jane Y. Wu, Kaili Liu, Weichuan Mo, Rongqiao He

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

miR-196a is located in the posterior trunk and plays a role in limb development. Here we show that miR-196a is able to induce eye anomaly in Xenopus laevis. Microinjection of synthetic miRNA precursor molecule for mammalian miR-196a into Xenopus embryo is sufficient for miR-196a overexpression during early development. The misexpression of miR-196a in anterior embryo led to dose-dependent eye anomalies, especially size reduction. In addition, the expression of ET, Rx1, Six3, Pax6, Lhx2, Optx2 and Ath5 in eye field or optic cup was also down-regulated. These results indicate that miR-196a can target gene(s) in the genetic network involved in eye formation, providing a potential tool for studying the mechanisms of eye development and diseases.

Original languageEnglish (US)
Pages (from-to)26-31
Number of pages6
JournalBrain Research Bulletin
Volume79
Issue number1
DOIs
StatePublished - Apr 6 2009

Keywords

  • Eye development
  • MicroRNA
  • Xenopus laevis
  • miR-196a

ASJC Scopus subject areas

  • Neuroscience(all)

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