Misexpression of miR-196a induces eye anomaly in Xenopus laevis

Rong Qiu; Ying Liu; Jane Y. Wu; Kaili Liu; Weichuan Mo; Rongqiao He

doi:10.1016/j.brainresbull.2008.12.009

Misexpression of miR-196a induces eye anomaly in Xenopus laevis

Rong Qiu, Ying Liu^*, Jane Y. Wu, Kaili Liu, Weichuan Mo, Rongqiao He

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

miR-196a is located in the posterior trunk and plays a role in limb development. Here we show that miR-196a is able to induce eye anomaly in Xenopus laevis. Microinjection of synthetic miRNA precursor molecule for mammalian miR-196a into Xenopus embryo is sufficient for miR-196a overexpression during early development. The misexpression of miR-196a in anterior embryo led to dose-dependent eye anomalies, especially size reduction. In addition, the expression of ET, Rx1, Six3, Pax6, Lhx2, Optx2 and Ath5 in eye field or optic cup was also down-regulated. These results indicate that miR-196a can target gene(s) in the genetic network involved in eye formation, providing a potential tool for studying the mechanisms of eye development and diseases.

Original language	English (US)
Pages (from-to)	26-31
Number of pages	6
Journal	Brain Research Bulletin
Volume	79
Issue number	1
DOIs	https://doi.org/10.1016/j.brainresbull.2008.12.009
State	Published - Apr 6 2009

Funding

We are grateful to Prof. Giuseppeina Barsacchi's lab for generously providing plasmid for Six3 probe; William A. Harris/Christine Holt's labs for Lhx2 , Optx2 and Ath5 . We thank Yunfeng Luo and Xiumei Wang for technical assistance. This work was supported by the NSFC (Nos. 30771129, 90408021) and National Basic Research Program of China (973 Program 2005CB522804).

Keywords

Eye development
MicroRNA
Xenopus laevis
miR-196a

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.brainresbull.2008.12.009

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title = "Misexpression of miR-196a induces eye anomaly in Xenopus laevis",

abstract = "miR-196a is located in the posterior trunk and plays a role in limb development. Here we show that miR-196a is able to induce eye anomaly in Xenopus laevis. Microinjection of synthetic miRNA precursor molecule for mammalian miR-196a into Xenopus embryo is sufficient for miR-196a overexpression during early development. The misexpression of miR-196a in anterior embryo led to dose-dependent eye anomalies, especially size reduction. In addition, the expression of ET, Rx1, Six3, Pax6, Lhx2, Optx2 and Ath5 in eye field or optic cup was also down-regulated. These results indicate that miR-196a can target gene(s) in the genetic network involved in eye formation, providing a potential tool for studying the mechanisms of eye development and diseases.",

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author = "Rong Qiu and Ying Liu and Wu, \{Jane Y.\} and Kaili Liu and Weichuan Mo and Rongqiao He",

note = "Funding Information: We are grateful to Prof. Giuseppeina Barsacchi's lab for generously providing plasmid for Six3 probe; William A. Harris/Christine Holt's labs for Lhx2 , Optx2 and Ath5 . We thank Yunfeng Luo and Xiumei Wang for technical assistance. This work was supported by the NSFC (Nos. 30771129, 90408021) and National Basic Research Program of China (973 Program 2005CB522804). ",

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doi = "10.1016/j.brainresbull.2008.12.009",

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AU - Qiu, Rong

AU - Liu, Ying

AU - Wu, Jane Y.

AU - Liu, Kaili

AU - Mo, Weichuan

AU - He, Rongqiao

N1 - Funding Information: We are grateful to Prof. Giuseppeina Barsacchi's lab for generously providing plasmid for Six3 probe; William A. Harris/Christine Holt's labs for Lhx2 , Optx2 and Ath5 . We thank Yunfeng Luo and Xiumei Wang for technical assistance. This work was supported by the NSFC (Nos. 30771129, 90408021) and National Basic Research Program of China (973 Program 2005CB522804).

PY - 2009/4/6

Y1 - 2009/4/6

N2 - miR-196a is located in the posterior trunk and plays a role in limb development. Here we show that miR-196a is able to induce eye anomaly in Xenopus laevis. Microinjection of synthetic miRNA precursor molecule for mammalian miR-196a into Xenopus embryo is sufficient for miR-196a overexpression during early development. The misexpression of miR-196a in anterior embryo led to dose-dependent eye anomalies, especially size reduction. In addition, the expression of ET, Rx1, Six3, Pax6, Lhx2, Optx2 and Ath5 in eye field or optic cup was also down-regulated. These results indicate that miR-196a can target gene(s) in the genetic network involved in eye formation, providing a potential tool for studying the mechanisms of eye development and diseases.

AB - miR-196a is located in the posterior trunk and plays a role in limb development. Here we show that miR-196a is able to induce eye anomaly in Xenopus laevis. Microinjection of synthetic miRNA precursor molecule for mammalian miR-196a into Xenopus embryo is sufficient for miR-196a overexpression during early development. The misexpression of miR-196a in anterior embryo led to dose-dependent eye anomalies, especially size reduction. In addition, the expression of ET, Rx1, Six3, Pax6, Lhx2, Optx2 and Ath5 in eye field or optic cup was also down-regulated. These results indicate that miR-196a can target gene(s) in the genetic network involved in eye formation, providing a potential tool for studying the mechanisms of eye development and diseases.

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KW - MicroRNA

KW - Xenopus laevis

KW - miR-196a

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Misexpression of miR-196a induces eye anomaly in Xenopus laevis

Abstract

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Keywords

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