Mitochondrial complex III ROS regulate adipocyte differentiation

Kathryn V. Tormos, Elena Anso, Robert B. Hamanaka, James Eisenbart, Joy Joseph, Balaraman Kalyanaraman, Navdeep S. Chandel*

*Corresponding author for this work

Research output: Contribution to journalArticle

372 Scopus citations

Abstract

Adipocyte differentiation is characterized by an increase in mitochondrial metabolism. However, it is not known whether the increase in mitochondrial metabolism is essential for differentiation or a byproduct of the differentiation process. Here, we report that primary human mesenchymal stem cells undergoing differentiation into adipocytes display an early increase in mitochondrial metabolism, biogenesis, and reactive oxygen species (ROS) generation. This early increase in mitochondrial metabolism and ROS generation was dependent on mTORC1 signaling. Mitochondrial-targeted antioxidants inhibited adipocyte differentiation, which was rescued by the addition of exogenous hydrogen peroxide. Genetic manipulation of mitochondrial complex III revealed that ROS generated from this complex is required to initiate adipocyte differentiation. These results indicate that mitochondrial metabolism and ROS generation are not simply a consequence of differentiation but are a causal factor in promoting adipocyte differentiation.

Original languageEnglish (US)
Pages (from-to)537-544
Number of pages8
JournalCell Metabolism
Volume14
Issue number4
DOIs
StatePublished - Oct 5 2011

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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    Tormos, K. V., Anso, E., Hamanaka, R. B., Eisenbart, J., Joseph, J., Kalyanaraman, B., & Chandel, N. S. (2011). Mitochondrial complex III ROS regulate adipocyte differentiation. Cell Metabolism, 14(4), 537-544. https://doi.org/10.1016/j.cmet.2011.08.007