Mitogenicity of a spread film of monophosphoryl lipid A

Gregory S. Retzinger*, Kuni Takayama

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

When spread at the air-water interface, monophosphoryl lipid A (MPLA) forms stable insoluble monolayers that collapse at ∼55 dyn/cm. At collapse, the exclusion area of each molecule is ∼119 Å2, consistent with the cross-sectional area of the lipid's 6 acyl chains. The nominal thickness of such films is ∼22 Å, determined, presumably, by the length of the acyl chains. For biological modeling of MPLA films, a system was developed in which monolayers of the lipid are supported by monodisperse hydrophobic beads of microscopic dimensions. Beads coated with MPLA monolayers within which the nominal area of each molecule is approximately equivalent to the "take-off" area of the lipid at the air-water interface, 280 Å2, are mitogenic for spleen cells. Given the natural occurrence of lipid A in the bacterial cell wall as well as the inherent stability of lipid A films, it seems reasonable to assume that at least some of the biological activities attributed to the lipid derive from its presentation/operation at an interface, i.e., on a surface. We propose beads coated with adsorbed films of lipid A will prove useful tools for modeling the activities of the lipid both in vitro and in vivo, and for elucidating the surface dependency and structural requirements of those activities.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalExperimental and Molecular Pathology
Volume79
Issue number2
DOIs
StatePublished - Oct 1 2005

Keywords

  • Adsorbed film
  • Innate immunity
  • Lipid A
  • Lipopolysaccharide
  • Mitogenicity
  • Monophosphoryl lipid A
  • Surface properties

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Mitogenicity of a spread film of monophosphoryl lipid A'. Together they form a unique fingerprint.

  • Cite this