Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold

Michael G. Poirier*, John F. Marko

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

131 Scopus citations


Isolated newt (Notophthalmus viridescens) chromosomes were studied by using micromechanical force measurement during nuclease digestion. Micrococcal nuclease and short-recognition-sequence blunt-cutting restriction enzymes first remove the native elastic response of, and then to go on to completely disintegrate, single metaphase newt chromosomes. These experiments rule out the possibility that the mitotic chromosome is based on a mechanically contiguous internal non-DNA (e.g., protein) "scaffold"; instead, the mechanical integrity of the metaphase chromosome is due to chromatin itself. Blunt-cutting restriction enzymes with longer recognition sequences only partially disassemble mitotic chromosomes and indicate that chromatin in metaphase chromosomes is constrained by isolated chromatin-crosslinking elements spaced by ≈15 kb.

Original languageEnglish (US)
Pages (from-to)15393-15397
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number24
StatePublished - Nov 26 2002

ASJC Scopus subject areas

  • General


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