Mitotic Transcriptional Activation: Clearance of Actively Engaged Pol II via Transcriptional Elongation Control in Mitosis

Kaiwei Liang, Ashley R. Woodfin, Brian D. Slaughter, Jay R. Unruh, Andrew C. Box, Ryan A. Rickels, Xin Gao, Jeffrey S. Haug, Sue L. Jaspersen, Ali Shilatifard*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Although it is established that some general transcription factors are inactivated at mitosis, many details of mitotic transcription inhibition (MTI) and its underlying mechanisms are largely unknown. We have identified mitotic transcriptional activation (MTA) as a key regulatory step to control transcription in mitosis for genes with transcriptionally engaged RNA polymerase II (Pol II) to activate and transcribe until the end of the gene to clear Pol II from mitotic chromatin, followed by global impairment of transcription reinitiation through MTI. Global nascent RNA sequencing and RNA fluorescence in situ hybridization demonstrate the existence of transcriptionally engaged Pol II in early mitosis. Both genetic and chemical inhibition of P-TEFb in mitosis lead to delays in the progression of cell division. Together, our study reveals a mechanism for MTA and MTI whereby transcriptionally engaged Pol II can progress into productive elongation and finish transcription to allow proper cellular division.

Original languageEnglish (US)
Pages (from-to)435-445
Number of pages11
JournalMolecular cell
Volume60
Issue number3
DOIs
StatePublished - Nov 5 2015

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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