Mixture of N-carbamoyl-L-glutamate plus L-arginine can protect rats with liver cirrhosis from acute ammonia intoxication

Wook Hwan Kim, Hyelee Park, Chawon Yun, Hyeseong Cho, Sangduk Kim, Woon Ki Paik, Soung Hoo Jeon, Jae Ho Lee*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background/Aims: We earlier reported that N-carbamoyl-L-glutamate (CG) plus L-arginine (Arg) protected normal and 70% hepatectomized rats from intoxication by a lethal or sub-lethal dose of ammonium acetate, respectively. In the present study, the protective effect of these compounds on cirrhotic rats was assessed. Methods: CG plus Arg were administered prior to the injection of a sub-lethal dose of ammonium acetate into dimethylnitrosamine-induced cirrhotic rats. Control rats were given phosphate-buffered saline (PBS) instead of the mixture. The behavior of the rats was monitored until the time of sacrifice. Blood ammonia level, blood urea nitrogen (BUN) and liver carbamoylphosphate synthetase I (CPS I) activity were determined. Results: Pretreatment of rats with the mixture of CG plus Arg could significantly lower the blood ammonia level (P < 0.05), increase the activity of CPS I (P < 0.05), improve abnormal behavior associated with ammonia intoxication (P < 0.05), and increase BUN (P < 0.05), as compared with the PBS-injected control group. There were significantly close correlations between (1) the increase of CPS I activity; (2) the improvement of abnormal behavior; (3) the increase of BUN; and (4) the decrease of the blood ammonia level. Conclusions: A mixture of CG plus Arg could protect rats with liver cirrhosis from acute ammonia intoxication.

Original languageEnglish (US)
Pages (from-to)719-725
Number of pages7
JournalJournal of Hepatology
Volume35
Issue number6
DOIs
StatePublished - Dec 1 2001

Keywords

  • Carbamoylphosphate synthetase I
  • Hyper-ammonemia
  • Liver cirrhosis
  • N-carbamoyl-L-glutamate plus L-arginine

ASJC Scopus subject areas

  • Hepatology

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