Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes

Paul W. Burridge, Sebastian Diecke, Elena Matsa, Arun Sharma, Haodi Wu, Joseph C. Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

23 Scopus citations

Abstract

The generation of cardiomyocytes from human induced pluripotent stem cells (hiPSCs) provides a source of cells that accurately recapitulate the human cardiac pathophysiology. The application of these cells allows for modeling of cardiovascular diseases, providing a novel understanding of human disease mechanisms and assessment of therapies. Here, we describe a stepwise protocol developed in our laboratory for the generation of hiPSCs from patients with a specific disease phenotype, long-term hiPSC culture and cryopreservation, differentiation of hiPSCs to cardiomyocytes, and assessment of disease phenotypes. Our protocol combines a number of innovative tools that include a codon-optimized mini intronic plasmid (CoMiP), chemically defined culture conditions to achieve high efficiencies of reprogramming and differentiation, and calcium imaging for assessment of cardiomyocyte phenotypes. Thus, this protocol provides a complete guide to use a patient cohort on a testable cardiomyocyte platform for pharmacological drug assessment.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages119-130
Number of pages12
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1353
ISSN (Print)1064-3745

Keywords

  • Calcium Imaging
  • Cardiomyocytes
  • Disease modeling
  • Human induced pluripotent stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes'. Together they form a unique fingerprint.

Cite this