@inbook{b94bf708c7194a879a1996961a0461b0,
title = "Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes",
abstract = "The generation of cardiomyocytes from human induced pluripotent stem cells (hiPSCs) provides a source of cells that accurately recapitulate the human cardiac pathophysiology. The application of these cells allows for modeling of cardiovascular diseases, providing a novel understanding of human disease mechanisms and assessment of therapies. Here, we describe a stepwise protocol developed in our laboratory for the generation of hiPSCs from patients with a specific disease phenotype, long-term hiPSC culture and cryopreservation, differentiation of hiPSCs to cardiomyocytes, and assessment of disease phenotypes. Our protocol combines a number of innovative tools that include a codon-optimized mini intronic plasmid (CoMiP), chemically defined culture conditions to achieve high efficiencies of reprogramming and differentiation, and calcium imaging for assessment of cardiomyocyte phenotypes. Thus, this protocol provides a complete guide to use a patient cohort on a testable cardiomyocyte platform for pharmacological drug assessment.",
keywords = "Calcium Imaging, Cardiomyocytes, Disease modeling, Human induced pluripotent stem cells",
author = "Burridge, {Paul W.} and Sebastian Diecke and Elena Matsa and Arun Sharma and Haodi Wu and Wu, {Joseph C.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2014.",
year = "2014",
doi = "10.1007/7651_2015_196",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "119--130",
booktitle = "Methods in Molecular Biology",
}