Modern mass spectrometry for synthetic biology and structure-based discovery of natural products

Matthew T. Henke, Neil L. Kelleher*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Covering: up to 2016 In this highlight, we describe the current landscape for dereplication and discovery of natural products based on the measurement of the intact mass by LC-MS. Often it is assumed that because better mass accuracy (provided by higher resolution mass spectrometers) is necessary for absolute chemical formula determination (≤1 part-per-million), that it is also necessary for dereplication of natural products. However, the average ability to dereplicate tapers off at ∼10 ppm, with modest improvement gained from better mass accuracy when querying focused databases of natural products. We also highlight some recent examples of how these platforms are applied to synthetic biology, and recent methods for dereplication and correlation of substructures using tandem MS data. We also offer this highlight to serve as a brief primer for those entering the field of mass spectrometry-based natural products discovery.

Original languageEnglish (US)
Pages (from-to)942-950
Number of pages9
JournalNatural product reports
Volume33
Issue number8
DOIs
StatePublished - Aug 2016

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Modern mass spectrometry for synthetic biology and structure-based discovery of natural products'. Together they form a unique fingerprint.

Cite this