Modulation of bradykinin-induced intracellular Ca2+ oscillations in v-Ki-ras-transformed fibroblasts

T. Fu*, Z. L. Gu, Y. H. Xu

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Modulation of bradykinin (BK)-induced intracellular Ca2+ oscillations was investigated with single cell Ca2+ analysis in v-Ki-ras-transformed NIH3T3 fibroblasts. The Ca2+ oscillations were inhibited by the addition of a specific antagonist for subtype 2 of BK receptors (B2 receptor), not the antagonist for B1 receptor. Decrease of the extracellular Ca2+ concentration suppressed the [Ca2+](i) oscillations and application of thapsigargin dissipated the [Ca2+](i) oscillations. These findings suggest that the continuous activation of B2 receptor leading to the fluctuations of both Ca2+ influx which refills the internal Ca2+ stores, and Ca2+ mobilization from the internal stores, is essential to the occurrence of the [Ca2+](i) oscillations in these cells.

Original languageEnglish (US)
Pages (from-to)511-515
Number of pages5
JournalActa Pharmacologica Sinica
Volume15
Issue number6
StatePublished - Jan 1 1994

Keywords

  • bradykinin
  • calcium
  • fibroblasts
  • oncogenes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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