Modulation of expression of genes encoding nuclear proteins following exposure to JANUS neutrons or γ-rays

Gayle E. Woloschak*, Chin Mei Chang-Liu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Previous work has shown that exposure of cells to ionizing radiations causes modulation of a variety of genes, including those encoding c-fos, interleukin-1, tumor necrosis factor, cytoskeletal elements, and many more. The experiments reported herein were designed to examine the effects of either JANUS neutron or γ-ray exposure on expression of genes encoding nucleus-associated proteins (H4-histone, c-jun, c-myc, Rb, and p53). Cycling Syrian hamster embryo cells were irradiated with varying doses and dose rates of either JANUS fission-spectrum neutrons or γ-rays; after incubation of the cell cultures for l h following radiation exposure, mRNA was harvested and analyzed by Northern blot. Results revealed induction of transcripts for c-jun, H4-histone, and (to a lesser extent) Rb following γ-ray but not following neutron exposure. Interestingly, expression of c-myc was repressed following γ-ray but not following neutron exposure. Radiations at different doses and dose rates were compared for each of the genes studied.

Original languageEnglish (US)
Pages (from-to)169-175
Number of pages7
JournalCancer Letters
Volume97
Issue number2
DOIs
StatePublished - Nov 6 1995

Keywords

  • Gamma rays
  • Genes
  • JANUS neutrons
  • Nuclear protein

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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