Modulation of expression of genes encoding nuclear proteins following exposure to JANUS neutrons or γ-rays

Gayle E. Woloschak; Chin Mei Chang-Liu

doi:10.1016/0304-3835(95)03973-Z

Modulation of expression of genes encoding nuclear proteins following exposure to JANUS neutrons or γ-rays

Gayle E. Woloschak^*, Chin Mei Chang-Liu

^*Corresponding author for this work

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Previous work has shown that exposure of cells to ionizing radiations causes modulation of a variety of genes, including those encoding c-fos, interleukin-1, tumor necrosis factor, cytoskeletal elements, and many more. The experiments reported herein were designed to examine the effects of either JANUS neutron or γ-ray exposure on expression of genes encoding nucleus-associated proteins (H4-histone, c-jun, c-myc, Rb, and p53). Cycling Syrian hamster embryo cells were irradiated with varying doses and dose rates of either JANUS fission-spectrum neutrons or γ-rays; after incubation of the cell cultures for l h following radiation exposure, mRNA was harvested and analyzed by Northern blot. Results revealed induction of transcripts for c-jun, H4-histone, and (to a lesser extent) Rb following γ-ray but not following neutron exposure. Interestingly, expression of c-myc was repressed following γ-ray but not following neutron exposure. Radiations at different doses and dose rates were compared for each of the genes studied.

Original language	English (US)
Pages (from-to)	169-175
Number of pages	7
Journal	Cancer Letters
Volume	97
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(95)03973-Z
State	Published - Nov 6 1995

Funding

We thank the following people who provided us with cDNA clones: c-jun cDNA from Dr. W. Lamph (Salk Institute); Rb clone from Dr. Dryja (Harvard University); H4-histone clone from Drs. G. and J. Stein (University of Florida); C-$X and c-myc from American Type Culture Collection; and ~53 from Dr. A. Levine (State University of New York at Stony Brook). We also thank Ms. K. Bexson for excellent secretarial expertise, Mr. G. Holmblad for his assistance in all irradiations, and Drs. F. Collart, C. Giometti, R. Drmanac and M. Churchill for critical review of the manuscript. This work was supported by the US Department of Energy, Office of Health and Environmental Research, under Contract No. W-31-109-ENG-38.

Keywords

Gamma rays
Genes
JANUS neutrons
Nuclear protein

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(95)03973-Z

Cite this

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title = "Modulation of expression of genes encoding nuclear proteins following exposure to JANUS neutrons or γ-rays",

abstract = "Previous work has shown that exposure of cells to ionizing radiations causes modulation of a variety of genes, including those encoding c-fos, interleukin-1, tumor necrosis factor, cytoskeletal elements, and many more. The experiments reported herein were designed to examine the effects of either JANUS neutron or γ-ray exposure on expression of genes encoding nucleus-associated proteins (H4-histone, c-jun, c-myc, Rb, and p53). Cycling Syrian hamster embryo cells were irradiated with varying doses and dose rates of either JANUS fission-spectrum neutrons or γ-rays; after incubation of the cell cultures for l h following radiation exposure, mRNA was harvested and analyzed by Northern blot. Results revealed induction of transcripts for c-jun, H4-histone, and (to a lesser extent) Rb following γ-ray but not following neutron exposure. Interestingly, expression of c-myc was repressed following γ-ray but not following neutron exposure. Radiations at different doses and dose rates were compared for each of the genes studied.",

keywords = "Gamma rays, Genes, JANUS neutrons, Nuclear protein",

author = "Woloschak, {Gayle E.} and Chang-Liu, {Chin Mei}",

note = "Funding Information: We thank the following people who provided us with cDNA clones: c-jun cDNA from Dr. W. Lamph (Salk Institute); Rb clone from Dr. Dryja (Harvard University); H4-histone clone from Drs. G. and J. Stein (University of Florida); C-$X and c-myc from American Type Culture Collection; and ~53 from Dr. A. Levine (State University of New York at Stony Brook). We also thank Ms. K. Bexson for excellent secretarial expertise, Mr. G. Holmblad for his assistance in all irradiations, and Drs. F. Collart, C. Giometti, R. Drmanac and M. Churchill for critical review of the manuscript. This work was supported by the US Department of Energy, Office of Health and Environmental Research, under Contract No. W-31-109-ENG-38. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1995",

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doi = "10.1016/0304-3835(95)03973-Z",

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AU - Woloschak, Gayle E.

AU - Chang-Liu, Chin Mei

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PY - 1995/11/6

Y1 - 1995/11/6

N2 - Previous work has shown that exposure of cells to ionizing radiations causes modulation of a variety of genes, including those encoding c-fos, interleukin-1, tumor necrosis factor, cytoskeletal elements, and many more. The experiments reported herein were designed to examine the effects of either JANUS neutron or γ-ray exposure on expression of genes encoding nucleus-associated proteins (H4-histone, c-jun, c-myc, Rb, and p53). Cycling Syrian hamster embryo cells were irradiated with varying doses and dose rates of either JANUS fission-spectrum neutrons or γ-rays; after incubation of the cell cultures for l h following radiation exposure, mRNA was harvested and analyzed by Northern blot. Results revealed induction of transcripts for c-jun, H4-histone, and (to a lesser extent) Rb following γ-ray but not following neutron exposure. Interestingly, expression of c-myc was repressed following γ-ray but not following neutron exposure. Radiations at different doses and dose rates were compared for each of the genes studied.

AB - Previous work has shown that exposure of cells to ionizing radiations causes modulation of a variety of genes, including those encoding c-fos, interleukin-1, tumor necrosis factor, cytoskeletal elements, and many more. The experiments reported herein were designed to examine the effects of either JANUS neutron or γ-ray exposure on expression of genes encoding nucleus-associated proteins (H4-histone, c-jun, c-myc, Rb, and p53). Cycling Syrian hamster embryo cells were irradiated with varying doses and dose rates of either JANUS fission-spectrum neutrons or γ-rays; after incubation of the cell cultures for l h following radiation exposure, mRNA was harvested and analyzed by Northern blot. Results revealed induction of transcripts for c-jun, H4-histone, and (to a lesser extent) Rb following γ-ray but not following neutron exposure. Interestingly, expression of c-myc was repressed following γ-ray but not following neutron exposure. Radiations at different doses and dose rates were compared for each of the genes studied.

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Modulation of expression of genes encoding nuclear proteins following exposure to JANUS neutrons or γ-rays

Abstract

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