Plasminogen-plasmin system play important roles in regulating tumor invasion, tissue remodeling and neovascularization. We investigated the effect of various cytokines on fibrinolytic activity produced by T98G glioblastoma cells. Cytokines used were interleukin(IL)-la and b, IL-2, IL-3, IL-4, IL-6, tumor necrotic factor (TNF) a and b. Highest fibrinolytic activity measured by fibrin autography was observed in the conditioned medium of T98G cells treated by IL-la and TNFa. IL-4 and IL-6 produced a slight increase in fibrinolytic activity, while IL-2 and IL-3 had little effect. Dexamethasone decreased significantly the fibrinolytic activity. Zymographic analysis and ELISA showed that the increased fibrinolytic activity induced by IL-ls and TNFs were caused by increased urokinase-type plasminogen activator secretion, and that decreased activity induced by dexamethasone was caused by a decrease in urokinase-type plasminogen activator and an increase in type-1 plasminogen activator inhibitor secretion.
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