Molecular analyses define Va7.2-Ja33+ MAIT cell depletion in HIV Infection: A case-control study

James E. Ussher*, Prabhjeet Phalora, Patrick Gerard Cormac Cosgrove, Rachel F. Hannaway, Andri Rauch, Huldrych F. Gunthard, Philip Goulder, Rodney E. Phillips, Christian B. Willberg, Paul Klenerman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Mucosal-Associated invariant T (MAIT) cells are an abundant antibacterial innate-like lymphocyte population. There are conflicting reports as to their fate in HIV infection. The objective of this study was to determine whether MAIT cells are truly depleted in HIV infection. In this case-control study of HIV-positive patients and healthy controls, quantitative real-Time polymerase chain reaction was used to assess the abundance of messenger RNA (mRNA) and genomic DNA (gDNA) encoding the canonical MAIT cell T cell receptor (Va7.2-Ja33). Comparison was made with flow cytometry. Significant depletion of both Va7.2-Ja33 mRNA and gDNA was seen in HIV infection. Depletion of Va7.2+CD161++ T cells was confirmed by flow cytometry. In HIV infection, the abundance of Va7.2-Ja33 mRNA correlated most strongly with the frequency of Va7.2+CD161++ cells. No increase was observed in the frequency of Va7.2+CD161-cells among CD3+CD4-lymphocytes. MAIT cells are depleted from blood in HIV infection as confirmed by independent assays. Significant accumulation of a CD161-MAIT cell population is unlikely. Molecular approaches represent a suitable alternative to flow cytometry-based assays for tracking of MAIT cells in HIV and other settings.

Original languageEnglish (US)
Article numbere1134
JournalMedicine (United States)
Volume94
Issue number29
DOIs
StatePublished - Jul 1 2015

ASJC Scopus subject areas

  • Medicine(all)

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