Molecular cloning and adhesive properties of murine platelet/endothelial cell adhesion molecule 1

Yong Xie*, William A. Muller

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

We describe the isolation and characterization of a functional murine platelet/endothelial cell adhesion molecule (PECAM) 1 cDNA clone from a mouse lung library. At the nucleotide level, the coding sequence of murine PECAM-1 is 73% identical to human PECAM-1, and at the amino acid level, the sequence is 79% homologous to its human counterpart. Southern hybridization reveals that one copy of the gene exists in the mouse genome; Northern hybridization reveals a single mRNA species in mouse lung tissue. COS-7 and mouse L cells transfected with murine PECAM-1 expressed a 130-kDa glycoprotein on their surfaces that reacted with anti-murine PECAM-1 monoclonal antibody and comigrated on SDS/PAGE with human PECAM-1. Stable L-cell transfectants aggregate with each other in a PECAM-dependent, homophilic manner.

Original languageEnglish (US)
Pages (from-to)5569-5573
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number12
DOIs
StatePublished - Jun 15 1993

Keywords

  • CD31
  • Immunoglobulin gene superfamily
  • Leukocytes
  • Membrane protein

ASJC Scopus subject areas

  • General

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