Molecular cloning and expression of a pituitary-specific receptor for growth hormone-releasing hormone

Kelly E. Mayo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

328 Scopus citations

Abstract

A novel cDNA was isolated from rat pituitary mRNA using the polymerase chain reaction to amplify sequences encoding G protein-coupled receptors. The human homolog of this cDNA was isolated and expressed in human kidney 293 cells, and membrane fractions from these cells were found to bind human GH-releasing hormone (GHRH) with high affinity and specificity. GHRH also stimulates intracellular cAMP production in these transfected cells. The encoded receptor protein contains seven potential membrane-spanning domains, a hallmark of G protein-coupled receptors, and is homologous to previously identified receptors for secretin and vasoactive intestinal peptide, ligands that are related to GHRH. The rat GHRH receptor mRNA is expressed predominantly, if not exclusively, in the anterior pituitary gland, the major target for GHRH action. These results define a mechanism for cellular signaling by GHRH and provide the opportunity to examine the role of the GHRH receptor in growth abnormalities that involve the GH axis.

Original languageEnglish (US)
Pages (from-to)1734-1744
Number of pages11
JournalMolecular Endocrinology
Volume6
Issue number10
DOIs
StatePublished - Oct 1 1992

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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