Molecular cloning and expression of mouse placental lactogen i complementary deoxyribonucleic acid

Peter Colosi, Frank Talamantes, Daniel I.H. Linzer*

*Corresponding author for this work

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

The mouse midpregnancy lactogen or placental lactogen I (mPL-I) is encoded by a 1.0-kilobase mRNA that appears transiently during gestation, with maximal amounts accumulating in the placenta at day 10 of pregnancy. Several cDNA clones for mPL-I have been isolated from a λgt11 expression library constructed from day 10-placental RNA. The cDNA sequence indicates that mPL-I is synthesized as a 224 amino acid precursor, and is secreted as a 194 amino acid glycosylated hormone. The deduced amino acid sequence of mPL-I is highly homologous to the known members of the PRL family in the mouse, and hybridization analysis indicates that the mouse genome contains several mPL-I genes. Introduction of the mPL-I cDNA in an expression vector into cultured mouse cells results in the synthesis and secretion of glycosylated mPL-I protein that is recognized by anti-mPL-I antiserum and is biologically active.

Original languageEnglish (US)
Pages (from-to)767-776
Number of pages10
JournalMolecular Endocrinology
Volume1
Issue number11
DOIs
StatePublished - Nov 1987

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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