Molecular cloning and single-channel properties of the cyclic nucleotide-gated channel from catfish olfactory neurons

Evan H. Goulding; John Ngai; Richard H. Kramer; Suzanne Colicos; Richard Axel; Steven A. Siegelbaum; Andrew Chess

doi:10.1016/0896-6273(92)90107-O

Molecular cloning and single-channel properties of the cyclic nucleotide-gated channel from catfish olfactory neurons

Evan H. Goulding^*, John Ngai, Richard H. Kramer, Suzanne Colicos, Richard Axel, Steven A. Siegelbaum, Andrew Chess

^*Corresponding author for this work

Psychiatry and Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

190 Scopus citations

Abstract

We have cloned a functional cDNA encoding the cyclic nucleotide-gated channel selectively expressed in cat-fish olfactory sensory neurons. The cyclic nucleotidegated channels share sequence and structural features with the family of voltage-gated ion channels. This homology is most evident in transmembrane region S4, the putative voltage sensor domain, and the H5 domain, thought to form the channel pore. We have characterized the single-channel properties of the cloned catfish channel and compared these properties with the channel in native catfish olfactory sensory neurons. The channel is activated equally well by cAMP and cGMP, shows only a slight voltage dependence of gating, and exhibits a pH-and voltage-dependent subconductance state similar to that observed for the voltage-gated L-type calcium channel.

Original language	English (US)
Pages (from-to)	45-58
Number of pages	14
Journal	Neuron
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/0896-6273(92)90107-O
State	Published - Jan 1992

ASJC Scopus subject areas

General Neuroscience

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title = "Molecular cloning and single-channel properties of the cyclic nucleotide-gated channel from catfish olfactory neurons",

abstract = "We have cloned a functional cDNA encoding the cyclic nucleotide-gated channel selectively expressed in cat-fish olfactory sensory neurons. The cyclic nucleotidegated channels share sequence and structural features with the family of voltage-gated ion channels. This homology is most evident in transmembrane region S4, the putative voltage sensor domain, and the H5 domain, thought to form the channel pore. We have characterized the single-channel properties of the cloned catfish channel and compared these properties with the channel in native catfish olfactory sensory neurons. The channel is activated equally well by cAMP and cGMP, shows only a slight voltage dependence of gating, and exhibits a pH-and voltage-dependent subconductance state similar to that observed for the voltage-gated L-type calcium channel.",

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AU - Goulding, Evan H.

AU - Ngai, John

AU - Kramer, Richard H.

AU - Colicos, Suzanne

AU - Axel, Richard

AU - Siegelbaum, Steven A.

AU - Chess, Andrew

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AB - We have cloned a functional cDNA encoding the cyclic nucleotide-gated channel selectively expressed in cat-fish olfactory sensory neurons. The cyclic nucleotidegated channels share sequence and structural features with the family of voltage-gated ion channels. This homology is most evident in transmembrane region S4, the putative voltage sensor domain, and the H5 domain, thought to form the channel pore. We have characterized the single-channel properties of the cloned catfish channel and compared these properties with the channel in native catfish olfactory sensory neurons. The channel is activated equally well by cAMP and cGMP, shows only a slight voltage dependence of gating, and exhibits a pH-and voltage-dependent subconductance state similar to that observed for the voltage-gated L-type calcium channel.

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Molecular cloning and single-channel properties of the cyclic nucleotide-gated channel from catfish olfactory neurons

Abstract

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