Molecular cloning of mouse placental lactogen cDNA

L. L. Jackson; P. Colosi; F. Talamantes; D. I.H. Linzer

doi:10.1073/pnas.83.22.8496

Molecular cloning of mouse placental lactogen cDNA

L. L. Jackson, P. Colosi, F. Talamantes, D. I.H. Linzer

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage λgt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDA polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-II antiserum. The mPL-II cDNA clones hybridize to a 1.0-kilobase placental-specific mRNA. This mRNA, found in the fetal portion of the placenta, appears as early as day 10 of gestation and increases to a maximal level by day 12. The mPL-II cDNA nucleotide sequence has been determined. This sequence contains an open reading frame encoding a polypeptide of 222 amino acids with the amino-terminal 31 amino acids forming the signal sequence for secretion. The predicted secreted protein has 51% amino acid homology with mouse prolactin.

Original language	English (US)
Pages (from-to)	8496-8500
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	83
Issue number	22
DOIs	https://doi.org/10.1073/pnas.83.22.8496
State	Published - 1986

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.83.22.8496

Cite this

@article{b0f933df28f24eac8bd7b0c12ea9c404,

title = "Molecular cloning of mouse placental lactogen cDNA",

abstract = "We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage λgt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDA polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-II antiserum. The mPL-II cDNA clones hybridize to a 1.0-kilobase placental-specific mRNA. This mRNA, found in the fetal portion of the placenta, appears as early as day 10 of gestation and increases to a maximal level by day 12. The mPL-II cDNA nucleotide sequence has been determined. This sequence contains an open reading frame encoding a polypeptide of 222 amino acids with the amino-terminal 31 amino acids forming the signal sequence for secretion. The predicted secreted protein has 51% amino acid homology with mouse prolactin.",

author = "Jackson, {L. L.} and P. Colosi and F. Talamantes and Linzer, {D. I.H.}",

year = "1986",

doi = "10.1073/pnas.83.22.8496",

language = "English (US)",

volume = "83",

pages = "8496--8500",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "22",

}

TY - JOUR

T1 - Molecular cloning of mouse placental lactogen cDNA

AU - Jackson, L. L.

AU - Colosi, P.

AU - Talamantes, F.

AU - Linzer, D. I.H.

PY - 1986

Y1 - 1986

N2 - We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage λgt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDA polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-II antiserum. The mPL-II cDNA clones hybridize to a 1.0-kilobase placental-specific mRNA. This mRNA, found in the fetal portion of the placenta, appears as early as day 10 of gestation and increases to a maximal level by day 12. The mPL-II cDNA nucleotide sequence has been determined. This sequence contains an open reading frame encoding a polypeptide of 222 amino acids with the amino-terminal 31 amino acids forming the signal sequence for secretion. The predicted secreted protein has 51% amino acid homology with mouse prolactin.

AB - We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage λgt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDA polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-II antiserum. The mPL-II cDNA clones hybridize to a 1.0-kilobase placental-specific mRNA. This mRNA, found in the fetal portion of the placenta, appears as early as day 10 of gestation and increases to a maximal level by day 12. The mPL-II cDNA nucleotide sequence has been determined. This sequence contains an open reading frame encoding a polypeptide of 222 amino acids with the amino-terminal 31 amino acids forming the signal sequence for secretion. The predicted secreted protein has 51% amino acid homology with mouse prolactin.

UR - http://www.scopus.com/inward/record.url?scp=0022996726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022996726&partnerID=8YFLogxK

U2 - 10.1073/pnas.83.22.8496

DO - 10.1073/pnas.83.22.8496

M3 - Article

C2 - 3464966

AN - SCOPUS:0022996726

SN - 0027-8424

VL - 83

SP - 8496

EP - 8500

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 22

ER -

Molecular cloning of mouse placental lactogen cDNA

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this