Molecular cloning of mouse placental lactogen cDNA

L. L. Jackson, P. Colosi, F. Talamantes, D. I.H. Linzer

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage λgt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDA polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-II antiserum. The mPL-II cDNA clones hybridize to a 1.0-kilobase placental-specific mRNA. This mRNA, found in the fetal portion of the placenta, appears as early as day 10 of gestation and increases to a maximal level by day 12. The mPL-II cDNA nucleotide sequence has been determined. This sequence contains an open reading frame encoding a polypeptide of 222 amino acids with the amino-terminal 31 amino acids forming the signal sequence for secretion. The predicted secreted protein has 51% amino acid homology with mouse prolactin.

Original languageEnglish (US)
Pages (from-to)8496-8500
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number22
DOIs
StatePublished - Jan 1 1986

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