Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: Differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues

Charles T. Roberts; Stephen R. Lasky; William L. Lowe; William T. Seaman; Derek Leroith

doi:10.1210/mend-1-3-243

Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: Differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues

Charles T. Roberts^*, Stephen R. Lasky, William L. Lowe, William T. Seaman, Derek Leroith

^*Corresponding author for this work

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255 Scopus citations

Abstract

Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 373x2032;-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)⁺ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.

Original language	English (US)
Pages (from-to)	243-248
Number of pages	6
Journal	Molecular Endocrinology
Volume	1
Issue number	3
DOIs	https://doi.org/10.1210/mend-1-3-243
State	Published - Mar 1987

ASJC Scopus subject areas

Molecular Biology
Endocrinology

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title = "Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: Differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues",

abstract = "Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 373x2032;-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.",

author = "Roberts, {Charles T.} and Lasky, {Stephen R.} and Lowe, {William L.} and Seaman, {William T.} and Derek Leroith",

year = "1987",

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Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids : Differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues. / Roberts, Charles T.; Lasky, Stephen R.; Lowe, William L. et al.

In: Molecular Endocrinology, Vol. 1, No. 3, 03.1987, p. 243-248.

Research output: Contribution to journal › Article › peer-review

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AU - Roberts, Charles T.

AU - Lasky, Stephen R.

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AU - Seaman, William T.

AU - Leroith, Derek

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AB - Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 373x2032;-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.

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Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: Differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues

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