Molecular mapping Alzheimer’s disease: Maldi imaging of formalin-fixed, paraffin-embedded human hippocampal tissue

Andrea R. Kelley, George Perry*, Chloe Bethea, Rudolph J. Castellani, Stephan B.H. Bach

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A method for the molecular mapping of formalin-fixed, paraffin-embedded human hippocampal tissue affected by Alzheimer’s disease (AD) is presented. This approach utilizes imaging mass spectrometry (IMS) with matrix-assisted laser desorption/ionization (MALDI). The usefulness of this technique in comparing diseased versus nor mal tissue at the molecular level while continuing to maintain topological and morphological integrity is evident in the preliminary findings. The critical correlation of the deparaffination, washing, matrix deposition, and analysis steps in handling the tissue sections and how these steps impact the successful mapping of human hippocampal tissue is clearly demonstrated. By use of this technique we have been able to identify several differences between the hippocampal AD tissue and the control hippocampal tissue. From the observed peptide clip masses we present preliminary identifications of the amyloid-beta peptides known to be prominent in the brains of those with AD. We have obtained high-resolution mass spectra and mass images with 100μm spatial resolution. Future experiments will couple this work with MALDI LIFT experiments to enable top down proteomics of fresh frozen tissue, which is not possible with paraffin-embedded tissues.

Original languageEnglish (US)
Pages (from-to)88-98
Number of pages11
JournalOpen Neurology Journal
Volume10
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

Keywords

  • Alzheimer’s disease
  • Amyloid-beta
  • Hippocampal tissue
  • MALDI imaging
  • Paraffin embedded tissue

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health

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