Molecular mass estimation by PFG NMR spectroscopy

Christopher A. Crutchfield, Douglas J. Harris*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


A simplified PFG NMR diffusion analysis method was developed to estimate the molecular mass of small molecules in dilute aqueous and organic solutions. Internal referencing was utilized to improve the experimental robustness and simplify the data analysis. Specifically, tetramethylsilane (TMS) and HDO were chosen for the organic and aqueous reference molecules, respectively. Relative diffusivity-molecular mass correlations were empirically developed in the range of 2-1280 g/mol for dilute CDCl3 and D2O solutions. The median error in the predicted molecular mass was found to be 10 rel%. The utility of the method was demonstrated by analyzing the major and minor components in olive oil.

Original languageEnglish (US)
Pages (from-to)179-182
Number of pages4
JournalJournal of Magnetic Resonance
Issue number1
StatePublished - Mar 2007


  • Diffusion
  • DOSY
  • Molecular mass
  • Relative diffusivity

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics


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