Molecular mechanism of cyclic-nucleotide-gated channel activation

Evan H. Goulding*, Gareth R. Tibbs, Steven A. Siegelbaum

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

172 Scopus citations

Abstract

STUDIES on the activation of ligand- and voltage-gated ion channels have identified regions involved in both ligand binding1 and voltage sensing2, but relatively little is known about how such domains are coupled to channel opening. Here we investigate the structural basis for the activation of cyclic-nucleotide-gated channels, which are directly opened by cytoplasmic cyclic nucleotides3,4 but are structurally homologous to voltage-gated channels5-7. By constructing chimaeras between cyclic-nucleotide-gated channels cloned from bovine retinal photoreceptors 8 and catfish olfactory neurons7, we identify two distinct domains that are important for ligand binding and channel gating. A putative α-helix in the carboxy-terminal binding domain determines the selectivity of the channel for activation by cGMP relative to cAMP. A domain in the amino-terminal region determines the ease with which channels open and thus influences agonist efficacy. We propose that channel opening is coupled to an allosteric conformational change in the binding site which enhances agonist binding. Thus, cyclic nucleotides activate the channel by binding tightly to the open state and stabilizing it.

Original languageEnglish (US)
Pages (from-to)369-374
Number of pages6
JournalNature
Volume372
Issue number6504
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • General

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