Molecular regulation of histone H3 trimethylation by COMPASS and the regulation of gene expression

Jessica Schneider; Adam Wood; Jung Shin Lee; Rebecca Schuster; Jeff Dueker; Courtney Maguire; Selene K. Swanson; Laurence Florens; Michael P. Washburn; Ali Shilatifard

doi:10.1016/j.molcel.2005.07.024

Molecular regulation of histone H3 trimethylation by COMPASS and the regulation of gene expression

Jessica Schneider, Adam Wood, Jung Shin Lee, Rebecca Schuster, Jeff Dueker, Courtney Maguire, Selene K. Swanson, Laurence Florens, Michael P. Washburn, Ali Shilatifard^*

^*Corresponding author for this work

Biochemistry and Molecular Genetics

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210 Scopus citations

Abstract

The Set1-containing complex COMPASS, which is the yeast homolog of the human MLL complex, is required for mono-, di-, and trimethylation of lysine 4 of histone H3. We have performed a comparative global proteomic screen to better define the role of COMPASS in histone trimethylation. We report that both Cps60 and Cps40 components of COMPASS are required for proper histone H3 trimethylation, but not for proper regulation of telomere-associated gene silencing. Purified COMPASS lacking Cps60 can mono- and dimethylate but is not capable of trimethylating H3^K4. Chromatin immunoprecipitation (ChIP) studies indicate that the loss subunits of COMPASS required for histone trimethylation do not affect the localization of Set1 to chromatin for the genes tested. Collectively, our results suggest a molecular requirement for several components of COMPASS for proper histone H3 trimethylation and regulation of telomere-associated gene expression, indicating multiple roles for different forms of histone methylation by COMPASS.

Original language	English (US)
Pages (from-to)	849-856
Number of pages	8
Journal	Molecular cell
Volume	19
Issue number	6
DOIs	https://doi.org/10.1016/j.molcel.2005.07.024
State	Published - Sep 16 2005

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molcel.2005.07.024

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title = "Molecular regulation of histone H3 trimethylation by COMPASS and the regulation of gene expression",

abstract = "The Set1-containing complex COMPASS, which is the yeast homolog of the human MLL complex, is required for mono-, di-, and trimethylation of lysine 4 of histone H3. We have performed a comparative global proteomic screen to better define the role of COMPASS in histone trimethylation. We report that both Cps60 and Cps40 components of COMPASS are required for proper histone H3 trimethylation, but not for proper regulation of telomere-associated gene silencing. Purified COMPASS lacking Cps60 can mono- and dimethylate but is not capable of trimethylating H3K4. Chromatin immunoprecipitation (ChIP) studies indicate that the loss subunits of COMPASS required for histone trimethylation do not affect the localization of Set1 to chromatin for the genes tested. Collectively, our results suggest a molecular requirement for several components of COMPASS for proper histone H3 trimethylation and regulation of telomere-associated gene expression, indicating multiple roles for different forms of histone methylation by COMPASS.",

author = "Jessica Schneider and Adam Wood and Lee, {Jung Shin} and Rebecca Schuster and Jeff Dueker and Courtney Maguire and Swanson, {Selene K.} and Laurence Florens and Washburn, {Michael P.} and Ali Shilatifard",

note = "Funding Information: The authors are grateful to Mrs. Kristen Tenney for critically reading the manuscript. This work was supported in part by a grant from the National Institutes of Health (1R01GM069905) to A.S. A.S. is a Scholar of the Leukemia and Lymphoma Society.",

year = "2005",

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doi = "10.1016/j.molcel.2005.07.024",

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Molecular regulation of histone H3 trimethylation by COMPASS and the regulation of gene expression. / Schneider, Jessica; Wood, Adam; Lee, Jung Shin; Schuster, Rebecca; Dueker, Jeff; Maguire, Courtney; Swanson, Selene K.; Florens, Laurence; Washburn, Michael P.; Shilatifard, Ali.

In: Molecular cell, Vol. 19, No. 6, 16.09.2005, p. 849-856.

Research output: Contribution to journal › Article › peer-review

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AU - Schneider, Jessica

AU - Wood, Adam

AU - Lee, Jung Shin

AU - Schuster, Rebecca

AU - Dueker, Jeff

AU - Maguire, Courtney

AU - Swanson, Selene K.

AU - Florens, Laurence

AU - Washburn, Michael P.

AU - Shilatifard, Ali

N1 - Funding Information: The authors are grateful to Mrs. Kristen Tenney for critically reading the manuscript. This work was supported in part by a grant from the National Institutes of Health (1R01GM069905) to A.S. A.S. is a Scholar of the Leukemia and Lymphoma Society.

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N2 - The Set1-containing complex COMPASS, which is the yeast homolog of the human MLL complex, is required for mono-, di-, and trimethylation of lysine 4 of histone H3. We have performed a comparative global proteomic screen to better define the role of COMPASS in histone trimethylation. We report that both Cps60 and Cps40 components of COMPASS are required for proper histone H3 trimethylation, but not for proper regulation of telomere-associated gene silencing. Purified COMPASS lacking Cps60 can mono- and dimethylate but is not capable of trimethylating H3K4. Chromatin immunoprecipitation (ChIP) studies indicate that the loss subunits of COMPASS required for histone trimethylation do not affect the localization of Set1 to chromatin for the genes tested. Collectively, our results suggest a molecular requirement for several components of COMPASS for proper histone H3 trimethylation and regulation of telomere-associated gene expression, indicating multiple roles for different forms of histone methylation by COMPASS.

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Molecular regulation of histone H3 trimethylation by COMPASS and the regulation of gene expression

Abstract

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