Molecular subclassification of kidney tumors and the discovery of new diagnostic markers

Masayuki Takahashi, Ximing J. Yang, Jun Sugimura, Jesper Backdahl, Maria Tretiakova, Chao Nan Qian, Steven G. Gray, Robert Knapp, John Anema, Richard Kahnoski, David Nicol, Nicholas J. Vogelzang, Kyle A. Furge, Hiroomi Kanayama, Susumu Kagawa, Bin Tean Teh*

*Corresponding author for this work

Research output: Contribution to journalArticle

180 Scopus citations

Abstract

We analysed the expression profiles of 70 kidney tumors of different histological subtypes to determine if these subgroups can be distinguished by their gene expression profiles, and to gain insights into the molecular mechanisms underlying each subtype. In all, 39 clear cell renal cell carcinomas (RCC), seven primary and one metastatic papillary RCC, six granular RCC from old classification, five chromophobe RCC, five sarcomatoid RCC, two oncocytomas, three transitional cell carcinomas (TCC) of the renal pelvis and five Wilms' tumors were compared with noncancerous kidney tissues using microarrays containing 19 968 cDNAs. Based on global gene clustering of 3560 selected cDNAs, we found distinct molecular signatures in clear cell, papillary, chromophobe RCC/ oncocytoma, TCC and Wilms' subtypes. The close clustering in each of these subtypes points to different tumorigenic pathways as reflected by their histological characteristics. In the clear cell RCC clustering, two subgroups emerged that correlated with clinical outcomes, confirming the potential use of gene expression signatures as a predictor of survival. In the so-called granular cell RCC (terminology for a subtype that is no longer preferred), none of the six cases clusters together, supporting the current view that they do not represent a single entity. Blinded histological re-evaluation of four cases of 'granular RCC' led to their reassignment to other existing histological subtypes, each compatible with our molecular classification. Finally, we found gene sets specific to each subtype. In order to establish the use of some of these genes as novel subtype markers, we selected four genes and performed immunohistochemical analysis on 40 cases of primary kidney tumors. The results were consistent with the gene expression micro-array data: glutathione S-transferase α was highly expressed in clear cell RCC, α methylacyl racemase in papillary RCC, carbonic anhydrase II in chromophobe RCC and K19 in TCC. In conclusion, we demonstrated that molecular profiles of kidney cancers closely correlated with their histological subtypes. We have also identified in these subtypes differentially expressed genes that could have important diagnostic and therapeutic implications.

Original languageEnglish (US)
Pages (from-to)6810-6818
Number of pages9
JournalOncogene
Volume22
Issue number42 REV. ISS. 4
StatePublished - Sep 29 2003

Keywords

  • Diagnosis
  • Gene expression profiling
  • Kidney tumor
  • Microarray
  • Molecular classification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Takahashi, M., Yang, X. J., Sugimura, J., Backdahl, J., Tretiakova, M., Qian, C. N., Gray, S. G., Knapp, R., Anema, J., Kahnoski, R., Nicol, D., Vogelzang, N. J., Furge, K. A., Kanayama, H., Kagawa, S., & Teh, B. T. (2003). Molecular subclassification of kidney tumors and the discovery of new diagnostic markers. Oncogene, 22(42 REV. ISS. 4), 6810-6818.