Molecular typing for investigating an outbreak of Candida krusei

Gary A. Noskin*, Jenny Lee, Donna M. Hacek, Michael Postelnick, Boris E. Reisberg, Valentina Stosor, Sigmund A. Weitzman, Lance R. Peterson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Invasive infections due to Candida krusei are often observed in immunocompromised patients who have received prior therapy with fluconazole, although infection has also occurred in patients in the absence of this antifungal agent. From August 25 through September 19, 1995, we identified four patients with C. krusei fungemia on our hematology/oncology unit. Molecular typing of all the isolates was performed by restriction endonuclease analysis of genomic DNA using HinfI. A total of 7 patients found to be colonized or infected with C. krusei were matched with 14 controls. There was no difference between the cases and controls with respect to underlying disease, duration of hospitalization, or neutropenia. The numbers of days of hyperalimentation, corticosteroids, and antibiotics were similar between both groups. The mean number of antibiotics was greater in the cases versus controls (5.0 versus 2.5; p = .003). There was no difference with respect to total dose or duration of fluconazole administration. Molecular typing of the isolates revealed that four had identical DNA banding patterns, plus another two that differed by one band and were considered related. Three historical strains were unrelated. In conclusion, this report demonstrates that molecular typing can be used to define clonality and, thereby, support increased infection control practices to eliminate such outbreaks when evidence of clonal spread is present.

Original languageEnglish (US)
Pages (from-to)117-123
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume26
Issue number3-4
DOIs
StatePublished - Nov 1 1996

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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