Abstract
Reagent-free electronic biosensors capable of analyzing disease markers directly in unprocessed body fluids will enable the development of simple & affordable devices for personalized healthcare monitoring. Here we report a powerful and versatile nucleic acid-based reagent-free electronic sensing system. The signal transduction is based on the kinetics of an electrode-tethered molecular pendulum—a rigid double stranded DNA with one of the strands displaying an analyte-binding aptamer and the other featuring a redox probe—that exhibits field-induced transport modulated by receptor occupancy. Using chronoamperometry, which enables the sensor to circumvent the conventional Debye length limitation, the binding of an analyte can be monitored as these species increase the hydrodynamic drag. The sensing platform demonstrates a low femtomolar quantification limit and minimal cross-reactivity in analyzing cardiac biomarkers in whole blood collected from patients with chronic heart failure.
| Original language | English (US) |
|---|---|
| Article number | e202213567 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 62 |
| Issue number | 20 |
| DOIs | |
| State | Published - May 8 2023 |
Funding
Research reported in this publication was supported by the Canadian Institutes of Health Research (grant no. FDN\u2010148415). The opinions, results and conclusions are solely the responsibility of the authors and no endorsement by the funding agencies is intended or inferred. We would also like to acknowledge Stefan Superina from the Ted Rogers Centre for Heart Research, Toronto, ON, Canada for his support with the patient samples. All correspondence and requests for materials should be made to S.O.K.
Keywords
- Aptamers
- Cardiac Diseases
- Molecular Pendulum
- Multiplexed Biosensing
- Reagent-Free Biosensing
ASJC Scopus subject areas
- Catalysis
- General Chemistry