Monitoring Cardiac Biomarkers with Aptamer-Based Molecular Pendulum Sensors

Alam Mahmud, Dingran Chang, Jagotamoy Das, Surath Gomis, Farid Foroutan, Jenise B. Chen, Laxman Pandey, Connor D. Flynn, Hanie Yousefi, Armin Geraili, Heather J. Ross, Edward H. Sargent, Shana O. Kelley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Reagent-free electronic biosensors capable of analyzing disease markers directly in unprocessed body fluids will enable the development of simple & affordable devices for personalized healthcare monitoring. Here we report a powerful and versatile nucleic acid-based reagent-free electronic sensing system. The signal transduction is based on the kinetics of an electrode-tethered molecular pendulum—a rigid double stranded DNA with one of the strands displaying an analyte-binding aptamer and the other featuring a redox probe—that exhibits field-induced transport modulated by receptor occupancy. Using chronoamperometry, which enables the sensor to circumvent the conventional Debye length limitation, the binding of an analyte can be monitored as these species increase the hydrodynamic drag. The sensing platform demonstrates a low femtomolar quantification limit and minimal cross-reactivity in analyzing cardiac biomarkers in whole blood collected from patients with chronic heart failure.

Original languageEnglish (US)
Article numbere202213567
JournalAngewandte Chemie - International Edition
Volume62
Issue number20
DOIs
StatePublished - May 8 2023

Keywords

  • Aptamers
  • Cardiac Diseases
  • Molecular Pendulum
  • Multiplexed Biosensing
  • Reagent-Free Biosensing

ASJC Scopus subject areas

  • General Chemistry
  • Catalysis

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