Monitoring single-cell infectivity from virus-particle nanoarrays fabricated by parallel dip-pen nanolithography

Rafael A. Vega*, Clifton K.F. Shen, Daniel Maspoch, Jessica G. Robach, Robert A. Lamb, Chad A. Mirkin

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

44 Scopus citations

Abstract

A novel approach to monitor single-cell infectivity from biologically active virus particles fabricated by the dip-pen nanolithography (DPN)-generated affinity templates, has been presented. The high-resolution of DPN has facilitated the generation of chemical templates small enough to site isolate and control the orientation of individual virus particles. It was shown that when antibodies are immobilized on Zn-carboxylate-rich nanopatterns they can be used to assemble viruses in an active state, and cell infectivity can be monitored by using fluorescence and an enhanced green fluorescent protein (EGFP). The approach can be extended to study important microbiological events, such as how the number of virus particles, their orientation, and their chemical immobilization and presentation affect cellular infectivity for single and small collections of virus particles.

Original languageEnglish (US)
Pages (from-to)1482-1485
Number of pages4
JournalSmall
Volume3
Issue number9
DOIs
StatePublished - Sep 2007

Keywords

  • Cell infection
  • Dip-pen nanolithography
  • Patterning
  • Templating
  • Viruses

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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