TY - JOUR
T1 - Monoclonal antibodies to connective tissue macromolecules
T2 - Type II collagen
AU - Linsenmayer, Thomas F.
AU - Hendrix, Mary J.C.
N1 - Funding Information:
*This is publication no. 791 of the Robert W. Lovett Memorial Group for the Study of Diseases Causing Deformities. We thank Drs. Jerome Gross, Elizabeth D. Hay and Irwin Konigsberg for invaluable discussions. Supported by NIH grants, EY02261; AM03564 and HD00143. TFL is recipient of Research Career Development Award AM00031 and MJCH Postdoctoral Fellowship HL05682.
PY - 1980/1/29
Y1 - 1980/1/29
N2 - A monoclonal antibody against chick type II collagen has been produced by lymphocyte-myeloma cell hybridization. The antibody, harvested either from spent medium of hybridoma cultures or from ascites fluid of hybridoma-containing mice, has an extremely high titer against type II collagen but shows no activity against type I. The antigenic site of the collagen seems to be located within the helical portion of native molecules. Using fluorescence histochemical procedures, the antibody can be used to localize type II collagen in sectioned material.
AB - A monoclonal antibody against chick type II collagen has been produced by lymphocyte-myeloma cell hybridization. The antibody, harvested either from spent medium of hybridoma cultures or from ascites fluid of hybridoma-containing mice, has an extremely high titer against type II collagen but shows no activity against type I. The antigenic site of the collagen seems to be located within the helical portion of native molecules. Using fluorescence histochemical procedures, the antibody can be used to localize type II collagen in sectioned material.
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U2 - 10.1016/0006-291X(80)90352-6
DO - 10.1016/0006-291X(80)90352-6
M3 - Article
C2 - 7356475
AN - SCOPUS:0019186089
SN - 0006-291X
VL - 92
SP - 440
EP - 446
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -