A monoclonal antibody against chick type II collagen has been produced by lymphocyte-myeloma cell hybridization. The antibody, harvested either from spent medium of hybridoma cultures or from ascites fluid of hybridoma-containing mice, has an extremely high titer against type II collagen but shows no activity against type I. The antigenic site of the collagen seems to be located within the helical portion of native molecules. Using fluorescence histochemical procedures, the antibody can be used to localize type II collagen in sectioned material.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jan 29 1980|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology