Monoclonal antibody based ELISAs for measurement of activins in biological fluids

Wai Lee T Wong*, Shaily J. Garg, Teresa Woodruff, Laura Bald, Brian Fendly, James A. Lofgren

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Two sensitive monoclonal antibody (MAb)-based enzyme-linked immunosorbent assays (ELISAs), one for activin A (homodimer of βA subunits) and one for activin B (homodimer of βB subunits) in plasma have been developed. The activin A ELISA had an effective range of 0.2-50 ng/ml while the activin B ELISA's range was 0.1-25 ng/ml in human serum. Both ELISAs were specific with <0.01% cross-reactivity with related hormones and follistatin (an activin binding protein), however the presence of recombinant human follistatin caused a decrease in measured level of activin A and B spiked human samples. The assay was linear across the standard curve range with intra- and interassay coefficients of variation were less than 15%. The level of activins in female serum range from 0.3 to 10.4 ng/ml. In summary, we have developed a reliable, convenient and rapid MAb-based enzyme immunoassay for determination of activin A and B levels in human serum which are also applicable for buffer, mouse and monkey serum matrices. This assay will be useful for studying the regulation and role of activin A and B in health and disease.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalJournal of Immunological Methods
Volume165
Issue number1
DOIs
StatePublished - Sep 27 1993

Keywords

  • Activin A
  • Activin B
  • ELISA, one step
  • Monoclonal antibody pair selection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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