Monodisperse lipoplex generation by integrated picoliter micro reactor and incubator

Albert Tsung Hsi Hsieh*, Adam Yuh Lin, Abraham P. Lee

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A non-viral and efficient carrier for the applications delivering drugs, plasmic DNA, shRNA for pharmaceutical and therapeutic applications is presented. The cationic lipid based liposomal carriers which has been the most attractive non-viral solution. The size of complexes of cationic lipid and DNA (lipoplex) dictates the efficiency of gene transfection. Moreover, the order of mixing of cationic lipid and DNA, mixing rate, and mixture incubation time are factors that determine the lipoplex sizes during sample preparation. We developed a Picoliter Micro Reactor and Incubator (PMRI) system to formulate monodisperse cationic lipids and DNA complexes. The monodisperse micro reactor and incubator was designed and fabricated based on the microfluidic droplet formation devices which developed by our lab and others. Compared with the conventional irreproducible lipoplex preparation using hand-shaking or vortexing processes for mixing, the PMRI system we developed for lipoplex formulation demonstrates the ability of rapid and uniformly mix cationic lipid and DNA simultaneously, and generates a narrower size distribution peak and smaller average size. The PMRI also enables the mixing and incubation integrated process and lipolex formulation reproducibility.

Original languageEnglish (US)
Title of host publicationProceedings of 2006 International Conference on Microtechnologies in Medicine and Biology
Pages158-161
Number of pages4
DOIs
StatePublished - 2006
Externally publishedYes
Event2006 International Conference on Microtechnologies in Medicine and Biology - Okinawa, Japan
Duration: May 9 2006May 12 2006

Publication series

NameProceedings of 2006 International Conference on Microtechnologies in Medicine and Biology

Conference

Conference2006 International Conference on Microtechnologies in Medicine and Biology
Country/TerritoryJapan
CityOkinawa
Period5/9/065/12/06

Keywords

  • Gene transfection
  • Lipoplex
  • Micro-droplet
  • Micro-incubator
  • Micro-reactor

ASJC Scopus subject areas

  • Biomedical Engineering
  • Electrical and Electronic Engineering
  • General Medicine

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