Monodisperse liposomal gene carrier formulation in picoliter micro-reactor for consistent and efficient gene delivery

We developed a picoliter micro reactor and incubator system to formulate monodisperse cationic lipids and DNA complexes. The monodisperse micro reactor and incubator was designed and fabricated based on the microfluidic droplet formation devices which developed by our lab and others. Compared with the conventional irreproducible lipoplex preparation using hand-shaking or vortexing processes for mixing, the microfluidic platform developed for lipoplex formulation demonstrates the ability to rapidly and uniformly mix cationic lipid and DNA, resulting in the generation of lipoplex with narrower size distribution and smaller average size. This system enables an integrated process with constant mixing and incubation, and reproducibility of the lipoplex formulation.