Abstract
Mononuclear phagocyte (MP) brain migration influence neuronal damage during HIV-1-associated dementia (HAD). We demonstrate that potassium channels, expressed in human monocyte-derived macrophages (MDM), are vital for MP movement through Boyden chemotactic chambers, an artificial blood-brain barrier and organotypic hippocampal brain slices. MDM migration is inhibited by voltage-and calcium-activated potassium channel blockers that include charybodotoxin, margatoxin, agatoxin and apamin. This is observed both in uninfected and HIV-1-infected MP. The results suggest that potassium channels affect MDM brain migration through altering cell volume and shape. Such mechanisms likely affect MP-induced neuronal destruction during HAD.
Original language | English (US) |
---|---|
Pages (from-to) | 40-54 |
Number of pages | 15 |
Journal | Journal of Neuroimmunology |
Volume | 122 |
Issue number | 1-2 |
DOIs | |
State | Published - 2002 |
Funding
This work was supported in part by NIH grants RO1 NS36126-01, PO1 NS31492-06, 2R37 NS36126-05A1 and 2RO1 NS34239-04A1.
Keywords
- Cell migration
- Charybodotoxin
- Confocal microscopy
- HIV-infected monocyte
- K channel
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology