TY - JOUR
T1 - Mononuclear phagocyte biophysiology influences brain transendothelial and tissue migration
T2 - Implication for HIV-1-associated dementia
AU - Chung, Induk
AU - Zelivyanskaya, Marina
AU - Gendelman, Howard E.
N1 - Funding Information:
This work was supported in part by NIH grants RO1 NS36126-01, PO1 NS31492-06, 2R37 NS36126-05A1 and 2RO1 NS34239-04A1.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002
Y1 - 2002
N2 - Mononuclear phagocyte (MP) brain migration influence neuronal damage during HIV-1-associated dementia (HAD). We demonstrate that potassium channels, expressed in human monocyte-derived macrophages (MDM), are vital for MP movement through Boyden chemotactic chambers, an artificial blood-brain barrier and organotypic hippocampal brain slices. MDM migration is inhibited by voltage-and calcium-activated potassium channel blockers that include charybodotoxin, margatoxin, agatoxin and apamin. This is observed both in uninfected and HIV-1-infected MP. The results suggest that potassium channels affect MDM brain migration through altering cell volume and shape. Such mechanisms likely affect MP-induced neuronal destruction during HAD.
AB - Mononuclear phagocyte (MP) brain migration influence neuronal damage during HIV-1-associated dementia (HAD). We demonstrate that potassium channels, expressed in human monocyte-derived macrophages (MDM), are vital for MP movement through Boyden chemotactic chambers, an artificial blood-brain barrier and organotypic hippocampal brain slices. MDM migration is inhibited by voltage-and calcium-activated potassium channel blockers that include charybodotoxin, margatoxin, agatoxin and apamin. This is observed both in uninfected and HIV-1-infected MP. The results suggest that potassium channels affect MDM brain migration through altering cell volume and shape. Such mechanisms likely affect MP-induced neuronal destruction during HAD.
KW - Cell migration
KW - Charybodotoxin
KW - Confocal microscopy
KW - HIV-infected monocyte
KW - K channel
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U2 - 10.1016/S0165-5728(01)00462-3
DO - 10.1016/S0165-5728(01)00462-3
M3 - Article
C2 - 11777542
AN - SCOPUS:0036149403
SN - 0165-5728
VL - 122
SP - 40
EP - 54
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
IS - 1-2
ER -
