TY - JOUR
T1 - Morphogenesis in skin is governed by discrete sets of differentially expressed microRNAs
AU - Yi, Rui
AU - O'Carroll, Dónal
AU - Pasolli, Hilda A.
AU - Zhang, Zhihong
AU - Dietrich, Fred S.
AU - Tarakhovsky, Alexander
AU - Fuchs, Elaine
N1 - Funding Information:
We are grateful to the colleagues who we cite in the text for providing us with various antibodies and reagents. We thank T. Tuschl, S. Pfeffer, E. Bernstein, A. Giraldez and D. Bartel for advice and discussion. We are also grateful to members of the Fuchs lab for their help and critical discussions for the work, and to L. Polak and N. Stokes for assistance in the Rockefeller University Laboratory Animal Research Center. D.O.’C. acknowledges the support of the Irvington Institute for Immunological Research and is their National Genetics Foundation fellow. E.F is an Investigator of the Howard Hughes Medical Institute. This work was supported by the Howard Hughes Medical Institute and in part by a grant from the US National Institutes of Health (AR050452).
PY - 2006/3
Y1 - 2006/3
N2 - During embryogenesis, multipotent progenitors within the single-layered surface epithelium differentiate to form the epidermis and its appendages. Here, we show that microRNAs (miRNAs) have an essential role in orchestrating these events. We cloned more than 100 miRNAs from skin and show that epidermis and hair follicles differentially express discrete miRNA families. To explore the functional significance of this finding, we conditionally targeted Dicer1 gene ablation in embryonic skin progenitors. Within the first week after loss of miRNA expression, cell fate specification and differentiation were not markedly impaired, and in the interfollicular epidermis, apoptosis was not markedly increased. Notably, however, developing hair germs evaginate rather than invaginate, thereby perturbing the epidermal organization. Here we characterize miRNAs in skin, the existence of which was hitherto unappreciated, and demonstrate their differential expression and importance in the morphogenesis of epithelial tissues within this vital organ.
AB - During embryogenesis, multipotent progenitors within the single-layered surface epithelium differentiate to form the epidermis and its appendages. Here, we show that microRNAs (miRNAs) have an essential role in orchestrating these events. We cloned more than 100 miRNAs from skin and show that epidermis and hair follicles differentially express discrete miRNA families. To explore the functional significance of this finding, we conditionally targeted Dicer1 gene ablation in embryonic skin progenitors. Within the first week after loss of miRNA expression, cell fate specification and differentiation were not markedly impaired, and in the interfollicular epidermis, apoptosis was not markedly increased. Notably, however, developing hair germs evaginate rather than invaginate, thereby perturbing the epidermal organization. Here we characterize miRNAs in skin, the existence of which was hitherto unappreciated, and demonstrate their differential expression and importance in the morphogenesis of epithelial tissues within this vital organ.
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U2 - 10.1038/ng1744
DO - 10.1038/ng1744
M3 - Article
C2 - 16462742
AN - SCOPUS:33644619021
VL - 38
SP - 356
EP - 362
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
IS - 3
ER -