Morphological analysis of schlemm’s canal in mice

Benjamin R. Thomson*, Susan E. Quaggin

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Immunofluorescent confocal microscopy is a powerful tool for analysis of the morphology and development of vascular and lymphatic tissues. Schlemm’s canal (SC) is a large, lymphatic-like vessel in the anterior chamber of the eye, which is essential for aqueous humor drainage required to maintain intraocular pressure and is sensitive to defects in blood and lymphatic vascular signaling pathways. Here, we describe a method to stain and quantify SC area and morphology in enucleated mouse eyes, providing a tool for understanding its development and function in small animal genetic or disease models.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages153-160
Number of pages8
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1846
ISSN (Print)1064-3745

Keywords

  • Confocal microscopy
  • Immunofluorescence
  • Immunostaining
  • Whole mount

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Thomson, B. R., & Quaggin, S. E. (2018). Morphological analysis of schlemm’s canal in mice. In Methods in Molecular Biology (pp. 153-160). (Methods in Molecular Biology; Vol. 1846). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8712-2_10