Abstract
We present an imaging and image analysis approach to quantify both the overall morphology and the spatial distribution of nitrifying guilds in mixed-culture, nitrifying biofilms enriched on K1 carriers. For image acquisition we used optical coherence tomography to non-destructively visualize the three dimensional structure of entire internal cavities and fluorescent in situ hybridization of carrier cryosections to visualize the spatial distribution of ammonia oxidizing bacteria and nitrite oxidizing relative to the overall biofilm structure. Determination of morphological parameters relies on the adaptation of current image analysis methods to accommodate the complex, curved geometry of MBBR carriers.
| Original language | English (US) |
|---|---|
| Title of host publication | WEFTEC 2016 - 89th Water Environment Federation Annual Technical Exhibition and Conference |
| Publisher | Water Environment Federation |
| Pages | 4546-4555 |
| Number of pages | 10 |
| ISBN (Electronic) | 9781510870475 |
| DOIs | |
| State | Published - Jan 1 2016 |
| Event | 89th Annual Water Environment Federation Technical Exhibition and Conference, WEFTEC 2016 - New Orleans, United States Duration: Sep 24 2016 → Sep 28 2016 |
Publication series
| Name | WEFTEC 2016 - 89th Water Environment Federation Annual Technical Exhibition and Conference |
|---|---|
| Volume | 5 |
Conference
| Conference | 89th Annual Water Environment Federation Technical Exhibition and Conference, WEFTEC 2016 |
|---|---|
| Country/Territory | United States |
| City | New Orleans |
| Period | 9/24/16 → 9/28/16 |
Funding
The authors gratefully acknowledge the staff at the Terrence J. O’Brien water reclamation plant for assistance with primary effluent collection. This project was funded by the Civil & Environmental engineering department at Northwestern University.
ASJC Scopus subject areas
- Ecological Modeling
- Environmental Chemistry
- Pollution
- Waste Management and Disposal
- Water Science and Technology