Morphological identification of melanopsin-expressing retinal ganglion cell subtypes in mice

Seul Ki Lee, Tiffany M. Schmidt*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are a relatively recently discovered class of photoreceptor. ipRGCs can be subdivided into at least five subtypes (M1-M5), each of which has a distinct complement of morphological and physiological properties. ipRGC subtypes can be identified morphologically based on a combination of dendritic morphology and immunostaining for a cell-type specific marker. In this chapter, we describe methods for conclusively identifying each of the five ipRGC subtypes through a combination of patch clamp electrophysiology, Neurobiotin filling, visualization of ipRGC dendrites, and immunostaining for the marker SMI-32.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages275-287
Number of pages13
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1753
ISSN (Print)1064-3745

Keywords

  • Dendritic stratification
  • Intrinsically photosensitive retinal ganglion cell
  • Melanopsin
  • Mouse
  • Opn4
  • Patch clamp electrophysiology
  • Retina
  • SMI-32
  • Subtypes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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