Motile properties of vimentin intermediate filament networks in living cells

Miri Yoon, Robert D. Moir, Veena Prahlad, Robert D. Goldman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

199 Scopus citations

Abstract

The motile properties of intermediate filament (IF) networks have been studied in living cells expressing vimentin tagged with green fluorescent protein (GFP-vimentin). In interphase and mitotic cells, GFP-vimentin is incorporated into the endogenous IF network, and accurately reports the behavior of IF. Time-lapse observations of interphase arrays of vimentin fibrils demonstrate that they are constantly changing their configurations in the absence of alterations in cell shape. Intersecting points of vimentin fibrils, or foci, frequently move towards or away from each other, indicating that the fibrils can lengthen or shorten. Fluorescence recovery after photobleaching shows that bleach zones across fibrils rapidly recover their fluorescence. During this recovery, bleached zones frequently move, indicating translocation of fibrils. Intriguingly, neighboring fibrils within a cell can exhibit different rates and directions of movement, and they often appear to extend or elongate into the peripheral regions of the cytoplasm. In these same regions, short filamentous structures are also seen actively translocating. All of these motile properties require energy, and the majority appear to be mediated by interactions of IF with microtubules and microfilaments.

Original languageEnglish (US)
Pages (from-to)147-157
Number of pages11
JournalJournal of Cell Biology
Volume143
Issue number1
DOIs
StatePublished - Oct 5 1998

Keywords

  • Green fluorescent protein
  • Intermediate filaments
  • Microfilaments
  • Microtubules
  • Vimentin

ASJC Scopus subject areas

  • Cell Biology

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