Mouse and human mesoangioblasts: Isolation and characterization from adult skeletal muscles

Mattia Quattrocelli, Giacomo Palazzolo, Ilaria Perini, Stefania Crippa, Marco Cassano, Maurilio Sampaolesi*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

31 Scopus citations

Abstract

Mesoangioblasts (MABs) are mesoderm-derived stem cells, associated with small vessels and originally described in the mouse embryonic dorsal aorta. Similar though not identical cells have been later identified and characterized from postnatal small vessels of skeletal muscle and heart. They have in common the expression of pericyte markers, the anatomical location, the ability to self-renew in culture, and to differentiate into various types of mesodermal lineages upon proper culture conditions. Currently, the developmental origin of MABs and the relationship with other muscle stem cells are not understood in detail and are the subject of active research. This chapter provides an outline of the latest techniques for isolation and characterization of adult MABs from human and mouse skeletal muscles.

Original languageEnglish (US)
Title of host publicationMyogenesis
Subtitle of host publicationMethods and Protocols
EditorsJoseph DiMario
Pages65-76
Number of pages12
DOIs
StatePublished - Jan 2 2012

Publication series

NameMethods in Molecular Biology
Volume798
ISSN (Print)1064-3745

Keywords

  • Cocultures
  • FACS
  • Mesoangioblasts
  • Mesodermal lineages
  • Muscle stem cells
  • Pericytes
  • Surface antigens

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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