Abstract
Microarray technology can facilitate simultaneous expression analysis of thousands of genes and assist in delineating cellular pathways involved in development or disease pathogenesis. Since public databases and commercial cDNA microarrays have an under-representation of eye-expressed genes, we generated over 3000 expressed sequence tags from three unamplified mouse eye/retina cDNA libraries. These eye-expressed genes were used to produce cDNA microarrays. Methodology for printing of slides, hybridization, scanning and data analysis has been optimized. The I-gene microarrays will be useful for establishing expression profiles of the mouse eye/retina and provide a resource for defining molecular pathways involved in development, aging and disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 463-470 |
| Number of pages | 8 |
| Journal | Vision Research |
| Volume | 42 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2002 |
Funding
We wish to thank Drs. Alan Mears, Alfred Hero, Debashish Ghosh, and the members of Sensory Gene Microarray advisory committee (Drs. Kate Barald, Peter Hitchcock, Bret Hughes, Dave Kohrman, Margaret Lomax, Sy Moroi, Chris Nosrat) for advice and discussions. Authors are grateful to Sean MacNee and Matt Studt for technical assistance, and Sharyn Ferrara for administrative assistance. This research was supported by UM institutional funds and grants from the National Institutes of Health (EY11115 (supplement), EY07961, EY07003 (core), EY08123), the Macula Vision Research Foundation, the Foundation Fighting Blindness, and Research to Prevent Blindness (RPB). W.B. is the recipient of a Senior Investigator Award and A.S. is recipient of a Lew R. Wasserman Merit Award from RPB.
Keywords
- Cellular pathways
- Expression analysis
- Functional genomics
- Gene profile
- Mouse eye mutants
- Retinal degeneration
ASJC Scopus subject areas
- Sensory Systems
- Ophthalmology