Mouse models of multiple sclerosis: Experimental autoimmune encephalomyelitis and theiler's virus-induced demyelinating disease

Derrick P. McCarthy, Maureen H. Richards, Stephen D. Miller*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

140 Scopus citations


Experimental autoimmune encephalomyelitis (EAE) and Theiler's Murine Encephalitis Virus-Induced Demyelinating Disease (TMEV-IDD) are two clinically relevant murine models of multiple sclerosis (MS). Like MS, both are characterized by mononuclear cell infiltration into the CNS and demyelination. EAE is induced by either the administration of myelin protein or peptide in adjuvant or by the adoptive transfer of encephalitogenic T cell blasts into naïve recipients. The relative merits of each of these protocols are compared. Depending on the type of question being asked, different mouse strains and peptides are used. Different disease courses are observed with different strains and different peptides in active EAE. These variations are also addressed. Additionally, issues relevant to clinical grading of EAE in mice are discussed. In addition to EAE induction, useful references for other disease indicators such as DTH, in vitro proliferation, and immunohistochemistry are provided. TMEV-IDD is a useful model for understanding the possible viral etiology of MS. This section provides detailed information on the preparation of viral stocks and subsequent intracerebral infection of mice. Additionally, virus plaque assay and clinical disease assessment are discussed. Recently, recombinant TMEV strains have been created for the study of molecular mimicry which incorporate various 30 amino acid myelin epitopes within the leader region of TMEV.

Original languageEnglish (US)
Title of host publicationAutoimmunity
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages21
ISBN (Print)9781607617198
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Active induction
  • Adoptive transfer
  • EAE
  • Emulsion
  • Encephalitogenic
  • Epitope spreading
  • Experimental autoimmune encephalomyelitis
  • MBP
  • MOG
  • Multiple sclerosis
  • Myelin
  • Neurodegeneration
  • PLP
  • Relapsing-remitting
  • T cell blasts
  • Theiler's murine encephalomyelitis virus-induced demyelinating disease
  • VP2
  • VP3

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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