Mouse placental lactogen-1: Ria and gestational profile in maternal serum

L. Ogren, J. N. Southard, P. Colosi, D. I. Linzer, F. Talamantes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

A RIA for mouse placental lactogen-I (mPL-I) was developed using recombinant mPL-I as the standard, radioligand, and antigen for antiserum production. Displacement curves for dilutions of serum and placental extracts from pregnant mice were parallel to the recombinant mPL-I standard curve. Serum from male and nonpregnant female mice and high concentrations of mouse PRL, GH, PL-II, proliferin, and proliferin-related protein did not cross-react in the assay. mPL-I appeared in maternal serum on day 6 of pregnancy. Its concentration remained low until day 8 and then increased to a very large peak on days 9-11 (maximum concentration, 8 μg/ml). The mPL-I concentration declined after day 11, but the hormone could be detected at low concentration in maternal serum until the end of pregnancy. On day 10 of pregnancy, the mPL-I concentration of maternal serum was correlated with litter size. Fractionation of serum from 10-day pregnant mice by size exclusion chromatography indicated the absence of high mol wt forms of mPL-I in the circulation.

Original languageEnglish (US)
Pages (from-to)2253-2257
Number of pages5
JournalEndocrinology
Volume125
Issue number5
DOIs
StatePublished - Nov 1989

ASJC Scopus subject areas

  • Endocrinology

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