Movement of plasma-membrane-associated clathrin spots along the microtubule cytoskeleton

Joshua Z. Rappoport, Bushra W. Taha, Sanford M. Simon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The current understanding of the role of plasma-membrane-associated clathrin suggests that clathrin-coated pits form at the sites of activated receptors and then, following internalization, the clathrin coat is rapidly shed. Utilizing total internal reflection fluorescence microscopy (TIR-FM), we have documented linear lateral motion of cell-surface-associated dsRed-clathrin spots parallel to the plasma membrane. Clathrin spot motility was observed in multiple cell lines (MDCK, CHO, Cos-7 and HeLa). In MDCK cells dsRed-clathrin spots moved along linear pathways up to 4μm in length with rates of approximately 0.8μm/s. Spots did not generally undergo internalization during movement. The motion of these puncta was coincident with the microtubule cytoskeleton, and depolymerization of microtubules reduced spot motility over 10-fold. Over-expression of the microtubule-associated protein tau-EGFP decreased spot run length by 40% without affecting the rate of movement. Thus dsRed-clathrin puncta move along the microtubule cytoskeleton parallel to the cell surface.

Original languageEnglish (US)
Pages (from-to)460-467
Number of pages8
JournalTraffic
Volume4
Issue number7
DOIs
StatePublished - Jul 1 2003

Keywords

  • Clathrin
  • Evanescent wave microscopy
  • Microtubules
  • Tau
  • Total internal reflection fluorescence microscopy (TIR-FM)

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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